Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila.
Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila.
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DOI：
10.1371/journal.pone.0157413
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Dang Y;Xu X;Shen Y;Hu M;Zhang M;Li L;Lv L;Li J
草鱼（Ctenopharyngodon idella）是中国重要的商业养殖草食性鱼类，但极易感染嗜水气单胞菌。在本研究中，我们对一个抗病家族标本的脾脏组织进行了从头RNA-Seq测序，这些标本被腹腔注射含有或不含嗜水单胞杆菌剂量的PBS。分别于0 h和4、8、12、24、48、72 h分别从对照组和实验组取样。从归一化cDNA文库中获得12218万条clean reads；它们被组装成425,260个contigs和191,795个转录本。其中，52,668个转录本被NCBI Nr数据库注释，41,347个注释转录本被划分为90个功能组。20,569个独特基因被划分为6大类，其中包括38个次要KEGG通路。2992个unigenes被用于分析差异表达基因（DEGs）。89个推测的deg与免疫系统有关，41个参与补体和凝血级联通路。本研究通过对C. idella基因组资源的表达谱分析，为先天免疫中涉及的补体和补体相关途径提供了见解。我们认为，补体及其相关基因在防御嗜水单胞菌感染过程中发挥了重要作用。免疫应答在细菌注射后4小时被激活，表明补体途径在细菌感染的早期被激活。该研究提高了我们对C. idella对细菌病原体的免疫反应机制的理解。
The grass carp (Ctenopharyngodon idella) is an important commercial farmed herbivorous fish species in China, but is susceptible to Aeromonas hydrophila infections. In the present study, we performed de novo RNA-Seq sequencing of spleen tissue from specimens of a disease-resistant family, which were given intra-peritoneal injections containing PBS with or without a dose of A. hydrophila. The fish were sampled from the control group at 0 h, and from the experimental group at 4, 8, 12, 24, 48 and 72 h. 122.18 million clean reads were obtained from the normalized cDNA libraries; these were assembled into 425,260 contigs and then 191,795 transcripts. Of those, 52,668 transcripts were annotated with the NCBI Nr database, and 41,347 of the annotated transcripts were assigned into 90 functional groups. 20,569 unigenes were classified into six main categories, including 38 secondary KEGG pathways. 2,992 unigenes were used in the analysis of differentially expressed genes (DEGs). 89 of the putative DEGs were related to the immune system and 41 of them were involved in the complement and coagulation cascades pathway. This study provides insights into the complement and complement-related pathways involved in innate immunity, through expression profile analysis of the genomic resources in C. idella. We conclude that complement and complement-related genes play important roles during defense against A. hydrophila infection. The immune response is activated at 4 h after the bacterial injections, indicating that the complement pathways are activated at the early stage of bacterial infection. The study has improved our understanding of the immune response mechanisms in C. idella to bacterial pathogens.