Maturation of rat mast cells. An electron microscope study.
Maturation of rat mast cells. An electron microscope study.
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DOI：
10.1083/jcb.31.3.563
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发表时间：
1966-12
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影响因子：
--
通讯作者：
Combs JW
中科院分区：
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作者：
Combs JW
电镜下对大鼠肥大细胞成熟过程的研究证实了光镜下对肥大细胞分化特征的一些解释。此外，肥大细胞分化颗粒的超微结构变化表明，颗粒形成始于高尔基液泡内直径约70 mµIn的致密颗粒的细化。这些前微粒似乎聚集在膜内并融合形成直径70至100微米的致密绳。这些致密的索嵌在细颗粒物质中，可能是通过粗内质网的颗粒周围膜和池池之间的直接连续性而添加到发育中的颗粒中。密集的绳状和细颗粒状物质似乎被直径约30微米的大量链所取代，这被认为是两个先前分离的成分的重组产物。在成熟的大鼠肥大细胞中观察到的致密、均匀颗粒的形成过程被解释为链的压实。肥大细胞颗粒的形成与其他颗粒的形成有相似之处，特别提到了兔多形核白细胞azurophil颗粒。本文讨论了肥大细胞颗粒形成的超微结构、组织化学和放射自显影特征之间的关系，并讨论了颗粒周围膜的意义。
Electron microscope study of rat mast cell maturation corroborates certain interpretations of features of mast cell differentiation based on light microscope studies. In addition, the ultrastructural variation observed in the granules of differentiating mast cells suggests that granule formation begins with the elaboration of dense granules about 70 mµ in diameter inside Golgi vacuoles. These progranules appear to aggregate inside a membrane and fuse to form dense cords 70 to 100 mµ in diameter. These dense cords are embedded in a finely granular material possibly added to the developing granule by direct continuity between perigranular membranes and cisternae of rough endoplasmic reticulum. The dense cords and finely granular material then appear to be replaced by a mass of strands about 30 mµ in diameter, thought to be a reorganization product of the two formerly separate components. A process interpreted as compaction of the strands completes the formation of the dense, homogeneous granules observed in mature rat mast cells. The similarity between mast cell granule formation and the elaboration of other granules is considered, with special reference to rabbit polymorphonuclear leukocyte azurophil granules. The relationships between the ultrastructural, histochemical, and radioautographic characteristics of mast cell granule formation are considered, and the significance of the perigranular membrane is discussed.