Structure and function of an essential component of the outer membrane protein assembly machine
Structure and function of an essential component of the outer membrane protein assembly machine
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DOI：
10.1126/science.1143993
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发表时间：
2007-08-17
期刊：
影响因子：
56.9
通讯作者：
Kahne, Daniel
中科院分区：
文献类型：
--
作者：
Kim, Seokhee;Malinverni, Juliana C.;Kahne, Daniel
整合型β-桶状蛋白存在于线粒体、叶绿体和革兰氏阴性菌的外膜中。组装这些蛋白的机器包含一种整合膜蛋白，在大肠杆菌中称为YaeT，它具有一个或多个多肽转运相关（POTRA）结构域。YaeT的周质片段的晶体结构揭示了POTRA结构域的折叠方式，并为POTRA结构域如何结合不同的肽序列提出了一个模型，这是一个处理众多β - 桶状蛋白前体的机器所必需的。对POTRA结构域缺失的分析表明了哪些是必需的，并提供了这种组装机器的空间组织视图。
Integral beta-barrel proteins are found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria. The machine that assembles these proteins contains an integral membrane protein, called YaeT in Escherichia coli, which has one or more polypeptide transport-associated (POTRA) domains. The crystal structure of a periplasmic fragment of YaeT reveals the POTRA domain fold and suggests a model for how POTRA domains can bind different peptide sequences, as required for a machine that handles numerous beta-barrel protein precursors. Analysis of POTRA domain deletions shows which are essential and provides a view of the spatial organization of this assembly machine.