U2AF35 is encoded by an essential gene clustered in an operon with RRM/cyclophilin in Caenorhabditis elegans.
U2AF35 is encoded by an essential gene clustered in an operon with RRM/cyclophilin in Caenorhabditis elegans.
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DOI：
10.1017/s1355838299982225
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发表时间：
1999-04
期刊：
影响因子：
4.5
通讯作者：
D. Zorio;T. Blumenthal
中科院分区：
文献类型：
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作者：
D. Zorio;T. Blumenthal
在大多数物种中，3‘剪接位点最初是通过两个亚基剪接因子U2AF与多嘧啶(Poly)链的相互作用识别的，导致U2 SnRNP募集到上游的分支点共识。相反，在秀丽线虫中，Poly(Y)链和分支点共有序列都缺失，显然被高度保守的U4CAG/R3‘剪接位点共有序列所取代。然而，线虫U2AF65与其哺乳动物和苍蝇同行非常相似，可能识别3‘剪接位点共识。本文报道了线虫U2AF35基因UAF-2的克隆。我们发现它缺乏一个可识别的RS结构域，在果蝇中，该结构域已被证明在RNA结合中发挥作用，但它在其C末端包含一个延伸的富含甘氨酸的伸展部分。UAF-2与CYP-13在一个操纵子上，CYP-13是一个编码亲环素的基因，其N端带有RRM结构域。我们通过RNA干扰证明了U2AF基因UAF-1(编码U2AF65)和UAF-2是生存所必需的，而CYP-13显然不是必需的。
In most species the 3' splice site is recognized initially by an interaction between the two-subunit splicing factor U2AF with the polypyrimidine (poly(Y)) tract that results in recruitment of the U2 snRNP to the branch-point consensus just upstream. In contrast, in Caenorhabditis elegans, both the poly(Y) tract and the branch-point consensus sequences are missing, apparently replaced by the highly conserved U4CAG/R 3' splice site consensus. Nevertheless C. elegans U2AF65 is very similar to its mammalian and fly counterparts and may recognize the 3' splice site consensus. Here we report the cloning of the C. elegans U2AF35 gene, uaf-2. We show that it lacks an identifiable RS domain, which, in flies, has been shown to play a role in RNA binding, but it contains an extended glycine-rich stretch at its C-terminus. uaf-2 is in an operon with cyp-13, a gene that encodes a cyclophilin with an RRM domain at its N-terminus. We demonstrate by RNA interference that both U2AF genes, uaf-1 (which encodes U2AF65) and uaf-2, are required for viability, whereas cyp-13 is apparently not.