Separation behavior of electron-beam curing derived acrylate-based monoliths
Separation behavior of electron-beam curing derived acrylate-based monoliths
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DOI：
10.1002/jssc.200700364
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发表时间：
2007-11-01
影响因子：
3.1
通讯作者：
Buchmeiser, Michael R.
中科院分区：
文献类型：
--
作者：
Bandari, Rajendar;Eisner, Christian;Buchmeiser, Michael R.
电子束（EB）固化衍生的整料材料由甲基丙烯酸乙酯（EMA）、正丙烷三丙烯酸酯（TNIPTA）、2-丙醇、1-十二烷醇和甲苯在3 mm X 100 mm id玻璃柱的范围内制备，施加22 kGy的总剂量用于固化。检查整体柱对于选定的丹磺酰化（DNS）-氨基酸以及亲环蛋白18的分离行为。将它们的分离性能与C18改性的二氧化硅基刚性棒（Chromoliths（R））的分离性能进行比较。在丹磺酰化氨基酸的分离中，在EB衍生柱上的保留时间减少，其中峰分辨率显著优于Chromolith（R）。这一发现归因于EB固化衍生的整料中较大比例的小孔（< 2.15 nm）。最后，EB固化衍生的整料已用于从粗细胞裂解混合物中分离亲环蛋白18。
Electron beam (EB) curing-derived monolith materials were prepared from ethyl methacrylate (EMA), trimethylolpropane triacrylate (TNIPTA), 2-propanol, 1-dodecanol, and toluene within the confines of 3 mm x 100 mm id glass columns, applying a total dose of 22 kGy for curing. Monolithic columns were checked for their separation behavior for selected dansylated (DNS)-amino acids as well as for cyclophilin 18. Their separation performance was compared to that of a C18-modified silica-based rigid rod (Chromoliths (R)). In the separation of dansylated amino acids, retention times were reduced on EB-derived columns, where the peak resolution was significantly better than on a Chromolith (R). This finding was attributed to a larger fraction of small pores (< 2.15 nm) in the EB curing-derived monoliths. Finally, EB curing-derived monoliths have been used to separate cyclophilin 18 from crude cell lysis mixtures.