HNK-1-REACTIVE NOVEL OLIGOSACCHARIDE, SULFATE-O-3GLCA-BETA-1-4XYL-BETA-1-(4-METHYLUMBELLIFERONE), SYNTHESIZED BY CULTURED HUMAN SKIN FIBROBLASTS
HNK-1-REACTIVE NOVEL OLIGOSACCHARIDE, SULFATE-O-3GLCA-BETA-1-4XYL-BETA-1-(4-METHYLUMBELLIFERONE), SYNTHESIZED BY CULTURED HUMAN SKIN FIBROBLASTS
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DOI：
10.1074/jbc.270.23.13794
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发表时间：
1995-06-09
影响因子：
4.8
通讯作者：
ENDO, M
中科院分区：
文献类型：
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作者：
SHIBATA, S;TAKAGAKI, K;ENDO, M
将4-甲基伞形酮基-β-D-木糖苷（Xyl-MU）加入到培养的人皮肤成纤维细胞的培养基中，孵育后，合并培养基，并通过凝胶过滤色谱法纯化培养基中Xyl-MU诱导的寡糖。除了先前报道的Xyl-MU衍生物如Gal-Gal-Xyl-MU、Gal-Xyl-MU、Sia-Gal-Xyl-MU、GlcA-Xyl-MU，和Xyl-Xyl-MU。使用凝胶过滤色谱法和高效液相色谱法纯化新型Xyl-MU衍生物，然后进行碳水化合物组成分析、酶消化、Smith降解和离子喷雾质谱分析。结果表明，该化合物为硫酸根-O-3GlcA beta 1-4Xyl beta 1-MU。该Xyl-MU诱导的寡糖的非还原末端的结构与人外周神经源糖脂的寡糖链的结构相同，与小鼠单克隆抗体HNK-1反应，并且该Xyl-MU诱导的寡糖也与HNK-1反应。这些结果表明，在结构上与神经组织合成的人外周神经源性糖脂相同并与细胞粘附相关的寡糖也由间充质细胞合成。
4-Methylumbelliferyl-beta-D-xyloside (Xyl-MU) was added to the medium of cultured human skin fibroblasts, After incubation, the culture medium was pooled, and the Xyl-MU-induced oligosaccharides in the medium were purified by gel filtration chromatography, A novel Xyl-MU derivative was obtained, in addition to the previously reported Xyl-MU derivatives such as Gal-Gal-Xyl-MU, Gal-Xyl-MU, Sia-Gal-Xyl-MU, GlcA-Xyl-MU, and Xyl-Xyl-MU. The novel Xyl-MU derivative was purified using gel-filtration chromatography and high performance Liquid chromatography and then subjected to carbohydrate composition analysis, enzymic digestion, Smith degradation, and ion spray mass spectrometric analysis. The results indicated that it was sulfate-O-3GlcA beta 1-4Xyl beta 1-MU. The structure of the nonreducing terminal of this Xyl-MU-induced oligosaccharide was the same as that of the oligosaccharide chain of a human peripheral nerve derived glycolipid, reactive with the mouse monoclonal antibody HNK-1, and this Xyl-MU-induced oligosaccharide also reacted with HNK-1. These results suggest that the oligosaccharide, which is structurally identical to that of human peripheral nerve-derived glycolipid synthesized by nervous tissue and related to cell adhesion, is synthesized also by mesenchymal cells.