Proteomics reveals potential biomarkers of seed vigor in sugarbeet
Proteomics reveals potential biomarkers of seed vigor in sugarbeet
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DOI：
10.1002/pmic.201000586
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发表时间：
2011-05-01
期刊：
影响因子：
3.4
通讯作者：
Job, Dominique
中科院分区：
文献类型：
--
作者：
Catusse, Julie;Meinhard, Juliane;Job, Dominique
为了揭示种子活力的生物标记物，这是决定作物产量的一个重要性状，对甜菜种子样品进行了比较蛋白质组学研究，这些样品由一种名为氢引发的活力处理和一种名为控制变质的老化处理产生。比较蛋白质组学显示，种子样本之间的蛋白质表现出相反的行为。因此，18种蛋白质在启动过程中上调，在老化过程中下调，并在老化种子启动时进一步显示上调，这意味着老化过程中这些斑点体积的下调在随后的启动时是可逆的。另有11个蛋白质表现出相反的行为，即对照种子在引发和老化过程中斑点体积分别减少和增加，老化种子引发时斑点体积减少。这些结果支持了几种代谢途径在种子活力中的作用，这些代谢途径涉及脂肪和淀粉动员、蛋白质合成或甲基循环。它们也证实了以前的研究，即乙醛酸酶、异柠檬酸裂解酶、蛋白质合成能力和脱落酸信号通路的组成可能是种子活力的贡献者。
To unravel biomarkers of seed vigor, an important trait conditioning crop yield, a comparative proteomic study was conducted with sugarbeet seed samples of varying vigor as generated by an invigoration treatment called hydropriming and an aging treatment called controlled deterioration. Comparative proteomics revealed proteins exhibiting contrasting behavior between seed samples. Thus, 18 proteins were up-regulated during priming and down-regulated during aging and further displayed an up-regulation upon priming of the aged seeds, meaning that down-regulation of these spot volumes during aging was reversible upon subsequent priming. Also, 11 proteins exhibited the converse behavior characterized by a decrease and an increase of the spot volumes during priming and aging of the control seeds, respectively, and a decrease in the spot volumes upon priming of the aged seeds. The results underpinned the role in seed vigor of several metabolic pathways involved in lipid and starch mobilization, protein synthesis or the methyl cycle. They also corroborate previous studies suggesting that the glyoxylate enzyme isocitrate lyase, the capacity of protein synthesis and components of abscisic acid signaling pathways are likely contributors of seed vigor.