Pseudomonas Diversity Within Urban Freshwaters
Pseudomonas Diversity Within Urban Freshwaters
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DOI：
10.3389/fmicb.2019.00195
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发表时间：
2019-02-15
影响因子：
5.2
通讯作者：
Putonti, Catherine
中科院分区：
文献类型：
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作者：
Batrich, Mary;Maskeri, Laura;Putonti, Catherine
淡水湖泊是细菌群落的家园，有1000多个相互依赖的物种。许多高通量16S rRNA基因序列调查提供了深入了解这些沃茨内发现的微生物类群。先前对密歇根湖沃茨的调查已经确定了淡水湖常见的细菌物种以及可能从城市环境引入的物种。我们从密歇根湖的芝加哥近岸沃茨采集样本，培养细菌分离株，以更仔细地观察其中发现的物种的遗传多样性。检测到的最丰富的属是假单胞菌属，其在淡水中的存在通常归因于雨水或径流。对15株密歇根湖假单胞菌菌株进行了全基因组测序，这些菌株代表8个物种和3个无法用命名物种解析的分离株。这些基因组专门检查基因编码的功能，这可能是有利的，在他们的城市环境。在其他已知的毒力因子和防御机制中，抗生素抗性在基因组注释中被鉴定并在实验室中被验证。我们还测试了密歇根湖假单胞菌菌株的铁载体生产和耐重金属汞和铜。正如这里提出的研究表明，各种各样的假单胞菌居住在密歇根湖的城市沿海沃茨。
Freshwater lakes are home to bacterial communities with 1000s of interdependent species. Numerous high-throughput 16S rRNA gene sequence surveys have provided insight into the microbial taxa found within these waters. Prior surveys of Lake Michigan waters have identified bacterial species common to freshwater lakes as well as species likely introduced from the urban environment. We cultured bacterial isolates from samples taken from the Chicago nearshore waters of Lake Michigan in an effort to look more closely at the genetic diversity of species found there within. The most abundant genus detected was Pseudomonas, whose presence in freshwaters is often attributed to storm water or runoff. Whole genome sequencing was conducted for 15 Lake Michigan Pseudomonas strains, representative of eight species and three isolates that could not be resolved with named species. These genomes were examined specifically for genes encoding functionality which may be advantageous in their urban environment. Antibiotic resistance, amidst other known virulence factors and defense mechanisms, were identified in the genome annotations and verified in the lab. We also tested the Lake Michigan Pseudomonas strains for siderophore production and resistance to the heavy metals mercury and copper. As the study presented here shows, a variety of pseudomonads have inhabited the urban coastal waters of Lake Michigan.