A simple and selective detection method for aristolochic acid in crude drugs using solid-phase extraction
A simple and selective detection method for aristolochic acid in crude drugs using solid-phase extraction
复制标题
固相萃取简单选择性检测药材中马兜铃酸的方法
DOI：
10.1007/s11418-012-0739-7
复制
发表时间：
2013
影响因子：
3.3
通讯作者：
山崎勝弘
中科院分区：
文献类型：
--
作者：
田上貴臣;武田章弘;淺田安紀子;青山愛倫;土井崇広;川口正美;梶村計志;沢辺善之;尾花裕孝;山崎勝弘
日本药典第16版描述了使用常规高效液相色谱法（HPLC）分析细辛根中马兜铃酸I（AA-I）的日本官方方法。对药材进行HPLC分析后，有时会出现AA-Ⅰ的干扰峰。需要一种选择性的分析方法来确定AA-I是否存在于原料药中。本研究建立了一种固相萃取-液相色谱/质谱联用（LC/MS）的选择性分析方法，可用于药材中AA-Ⅰ的鉴定和质量控制。
The official Japanese method for analyzing aristolochic acid I (AA-I) in Asiasarum root using conventional high-performance liquid chromatography (HPLC) is described in the Japanese Pharmacopoeia, Sixteenth Edition. Interfering peaks of AA-I sometimes appear after HPLC analysis of crude drugs. A selective analytical method is needed to determine definitively whether AA-I is present in crude drugs. In this study, we developed a selective method that combined solid-phase extraction and liquid chromatography/mass spectrometry (LC/MS) which may be useful for identifying AA-I in crude drugs and for quality control.