Protein blotting in uniform or gradient electric fields.
Protein blotting in uniform or gradient electric fields.
复制标题
在均匀或梯度电场中进行蛋白质印迹。
DOI：
10.1016/0003-2697(85)90080-6
复制
发表时间：
1985
影响因子：
2.9
通讯作者：
Palade,GE
中科院分区：
文献类型：
--
作者：
Gershoni,JM;Davis,FE;Palade,GE
利用实验数据和计算机绘图分析了蛋白质印迹仪中各种电极阵列产生的电场。不对称的电极阵列被发现产生不均匀的领域，影响不均匀的转移of125碘标记的白蛋白从凝胶到硝酸纤维素膜。具有多个（四个）独立的线电极的对称阵列，分别提供电流，产生最均匀的场并实现测试蛋白质的最均匀的转移。利用多个独立的电极，可以产生梯度电场，其中可以消除大蛋白和小蛋白之间的电泳洗脱差异。具有均匀或梯度电场的转移装置有望改善定性结果，并使蛋白质印迹定量成为可能。
Experimental data and computer mapping were used to analyze electric fields generated by a variety of electrode arrays in protein blotting apparatus. Asymmetric electrode arrays were found to generate nonuniform fields that effected uneven transfers of125I-labeled albumin from gels to nitrocellulose membranes. Symmetric arrays with multiple (four), independent wire electrodes, supplied individually with electric current, generated the most uniform fields and effected the most even transfers of the test protein. With multiple independent electrodes, gradient electric fields can be generated in which differences in electrophoretic elution between large and small proteins can be eliminated. Transfer apparatus with either uniform or gradient electric fields are expected to improve qualitative results and make possible quantitation of protein blotting.