SIMULTANEOUS GENERATION OF TUBERCULIN-TYPE AND CUTANEOUS BASOPHILIC HYPERSENSITIVITY AT SEPARATE SITES IN THE GUINEA-PIG
SIMULTANEOUS GENERATION OF TUBERCULIN-TYPE AND CUTANEOUS BASOPHILIC HYPERSENSITIVITY AT SEPARATE SITES IN THE GUINEA-PIG
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DOI：
10.1159/000232227
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
ASKENASE, PW
中科院分区：
文献类型：
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作者：
LEONARD, EJ;LETTBROWN, MA;ASKENASE, PW
2 株豚鼠接受了 2 次单独的皮内免疫。第一种是活 BCG（Phipps 株）和同系 10 系肝癌细胞的混合物。第二次是在 6 周或更长时间后施用，是盐水中的匙孔血蓝蛋白 (KLH)。第二次注射后一周，用肿瘤细胞、纯化的结核菌素蛋白衍生物(PPD)和KLH在不同部位对动物进行皮肤测试。迟发性皮肤过敏 (DCH) 反应在 24 小时内就很明显。此时，通过保留嗜碱性粒细胞形态和染色质量的组织学方法处理反应位点。不同抗原引发三种独特的浸润。除了所有 3 种反应共有的单核细胞浸润外，KLH 部位还具有高浓度的嗜碱性粒细胞和少量中性粒细胞，这是皮肤嗜碱性超敏反应 (CBH) 的特征。 PPD 部位几乎没有嗜碱性粒细胞，但有许多中性粒细胞。肿瘤细胞反应主要是单核细胞，有少量嗜碱性粒细胞，几乎没有中性粒细胞。在同一动物中同时产生不同的DCH反应和细胞浸润的定量描述为DCH分类提供了基础，并以KLH CBH和结核菌素反应作为比较标准。由于在同一动物的不同部位用 3 种抗原进行攻击会产生具有截然不同的细胞浸润模式的 DCH 反应，因此差异的原因必须发生在局部部位，或者如果是循环的话，则必须是抗原特异性的。
Strain-2 guinea pigs received 2 separate intradermal immunizations. The first was a mixture of living BCG (Phipps strain) and syngeneic line-10 hepatoma cells. The second, administered after 6 wk or more, was keyhole limpet hemocyanin (KLH) in saline. One week after the 2nd injection, the animals were skin tested at separate sites with tumor cells, purified protein derivative of tuberculin (PPD) and KLH. Delayed cutaneous hypersensitivity (DCH) reactions were evident by 24 h. At this time the reaction sites were processed by histological methods that preserved basophil morphology and staining qualities. Three distinctive infiltrates were elicited by the different antigens. In addition to the mononuclear cell infiltrate common to all 3 reactions, the KLH site had a high concentration of basophils and few neutrophils, characteristic of cutaneous basophilic hypersensitivity (CBH). The PPD site had practically no basophils but many neutrophils. The tumor cell reaction was predominantly mononuclear, with small numbers of basophils and virtually no neutrophils. Simultaneous generation of different DCH reactions in the same animal and quantitative description of the cellular infiltrate provided a basis for DCH classifications, with KLH CBH and tuberculin reactions as standards for comparison. Since challenge with 3 antigens at separate sites in the same animal generated DCH reactions with widely differing patterns of cellular infiltration, the reason for the differences must occur at the local sites or, if circulating, must be antigen-specific.