Corneal infections in mice with toxin A and elastase mutants of Pseudomonas aeruginosa.
Corneal infections in mice with toxin A and elastase mutants of Pseudomonas aeruginosa.
复制标题
小鼠角膜感染铜绿假单胞菌毒素 A 和弹性蛋白酶突变体。
DOI：
10.1093/infdis/142.4.547
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Iglewski,BH
中科院分区：
文献类型：
--
作者：
Ohman,DE;Burns,RP;Iglewski,BH
提供的数据表明，在小鼠角膜的实验性感染中，铜绿假单胞菌的A毒素与生物体的致病性有关，而活性弹性蛋白酶可能不是必需的。在创伤后，角膜被野生型亲本毒素A产生菌株、两个毒素A缺失突变体(Tox-)或一个弹性蛋白酶突变体感染。两个Tox突变体产生的感染都没有它们的亲本菌株产生的感染严重。此外，Tox突变体不能像它们的亲本菌株那样在眼睛里持续很长时间。将亚破坏性剂量的外源毒素A添加到受TOX：突变体PA103-29感染的眼睛中，显著增加了其毒力。弹力酶突变体的感染过程和由此造成的角膜损伤与其亲本菌株的感染过程和结果没有什么区别。
The data presented indicate that in experimental infections of the mouse cornea, toxin A ofPseudomonas aeruginosacontributes to the organism's pathogenicity, whereas active elastase may not be required. After traumatization, corneas were infected with wild-type parental toxin A-producing strains, two toxin A-deficient mutants (Tox-), or an elastase mutant. The infections produced by both Tox- mutants were less severe than infections produced by their parental strains. Furthermore, the Tox-mutants were not able to persist in the eyes as long as their parental strains. Addition of subdamaging doses of exogenous toxin A to eyes infected with the Tox: mutant PA103-29 significantly increased its virulence. The course of infection and the resulting corneal damage produced by the elastase mutant were indistinguishable from those of its parental strain.