Glutamate release from satellite glial cells of the murine trigeminal ganglion
Glutamate release from satellite glial cells of the murine trigeminal ganglion
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DOI：
10.1016/j.neulet.2014.06.047
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发表时间：
2014-08
影响因子：
2.5
通讯作者：
Lysann Wagner;R. Warwick;T. Pannicke;A. Reichenbach;A. Grosche;M. Hanani
中科院分区：
文献类型：
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作者：
Lysann Wagner;R. Warwick;T. Pannicke;A. Reichenbach;A. Grosche;M. Hanani
已有研究认为，谷氨酸是感觉神经节内神经元和卫星胶质细胞(SGCs)之间的媒介，SGCs释放谷氨酸。用一种新的方法，我们研究了小鼠三叉神经节SGCs的谷氨酸释放。从神经胶质细胞囊泡吐出受到抑制的野生型和转基因小鼠中，用酶法分离到贴壁SGCs的感觉神经元。微量荧光法检测胞外谷氨酸。在细胞中加入不耐光的钙离子螯合剂后，紫外线可使细胞内钙离子浓度升高，导致谷氨酸的释放。胞吐抑制的细胞和药物阻断后的细胞释放谷氨酸的量减少。结果表明，三叉神经节的SGCs以钙依赖的方式释放谷氨酸。
It has been proposed that glutamate serves as a mediator between neurons and satellite glial cells (SGCs) in sensory ganglia and that SGCs release glutamate. Using a novel method, we studied glutamate release from SGCs from murine trigeminal ganglia. Sensory neurons with adhering SGCs were enzymatically isolated from wild type and transgenic mice in which vesicular exocytosis was suppressed in glial cells. Extracellular glutamate was detected by microfluorimetry. After loading the cells with a photolabile Ca2+chelator, the intracellular Ca2+concentration was raised in SGCs by a UV pulse, which resulted in glutamate release. The amount of released glutamate was decreased in cells with suppressed exocytosis and after pharmacological block of hemichannels. The data demonstrate that SGCs of the trigeminal ganglion release glutamate in a Ca2+-dependent manner.