Development of microsatellite markers for the Asian Stubtail Urosphena sauarneiceas by using next-generation sequencing technology.
Development of microsatellite markers for the Asian Stubtail Urosphena sauarneiceas by using next-generation sequencing technology.
复制标题
利用下一代测序技术开发亚洲短尾尾尾藻的微卫星标记。
DOI：
10.1007/s12686-013-9960-2
复制
发表时间：
2013
影响因子：
1.1
通讯作者：
Inoue-Murayama M
中科院分区：
文献类型：
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作者：
Kamioki M;Ando H;Isagi Y;Inoue-Murayama M
亚洲StubtailUrosphena squameicepswinters在东南亚正面临着严重的威胁，森林砍伐和物种被归类为受威胁的物种在一些繁殖种群。监测该物种在该领域的种群动态是困难的，因为他们是秘密的，喜欢密集的灌木丛。因此，从该物种开发遗传标记是有用的，以检测适当的保护管理单位，并了解其独特的育种系统。利用新一代测序仪分离并鉴定了13个微卫星位点。每个位点的等位基因数和期望杂合度分别为2-26个（平均10.31）和0.14-0.92个（平均0.64）。组合的第一亲非排除概率<0.005。
The Asian StubtailUrosphena squameicepswinters in Southeast Asia which is facing a serious threat from deforestation and the species is categorized as a threatened species at some of the breeding populations. Monitoring population dynamics of the species in the field is difficult because they are secretive and prefer dense undergrowth. Therefore, developing genetic markers from the species is useful for detecting appropriate conservational management units and to understanding their unique breeding system. We isolated and characterized 13 microsatellite loci of the Asian Stubtail by using a next-generation sequencer. The number of alleles and the expected heterozygosity at each locus were 2–26 (mean 10.31) and 0.14–0.92 (mean 0.64), respectively. The combined first-parent non-exclusion probability was <0.005.