SIZE AND STRUCTURE OF THE HIGHLY REPETITIVE BAM HI ELEMENT IN MICE
SIZE AND STRUCTURE OF THE HIGHLY REPETITIVE BAM HI ELEMENT IN MICE
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DOI：
10.1093/nar/11.15.5073
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发表时间：
1983-01-01
影响因子：
14.9
通讯作者：
FANNING, TG
中科院分区：
文献类型：
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作者：
FANNING, TG
小鼠中的长散布DNA的BAM HI家族代表多达小鼠基因组的0.5%。通过限制性酶切图谱和DNA测序分析了含有BAM H1/非BAM H1连接序列的克隆小鼠DNA片段。已经发现BAM HI元件：（i）大小约为7个双链酶对，（ii）不被类似于前病毒末端重复的长重复序列包围，和（iii）在一端含有聚-dA轨道。这些数据有力地表明，BAM HI元素出现的过程中涉及RNA中间体。该元件的开始，与poly-dA轨道相对，含有22个碱基对的序列，与可能在DNA复制中起作用的普遍存在的哺乳动物序列具有65%的同源性（1）。元件的poly-dA末端含有BAM 5和R序列，这两种序列均已在先前描述（2，3）。
The BAM HI family of long interspersed DNAs in mice represent as much as 0.5% of the mouse genome. Cloned mouse DNA fragments which contain BAM Hl/non-BAM HI junction sequences have been analyzed by restriction mapping and DNA sequencing. It has been found that BAM HI elements: (i) are approximately 7 kilobase pairs in size, (ii) are not bracketed by long repeated sequences analogous to the terminal repeats of proviruses and (iii) contain a poly-dA track at one end. The data strongly suggest that BAM HI elements arose by a process involving RNA intermediates. The beginning of the element, opposite the poly-dA track, contains a 22 base pair sequence exhibiting 65% homology to a ubiquitous mammalian sequence which may play a role in DNA replication (1). The poly-dA end of the element contains BAM5 and R sequences, both of which have been described previously (2,3).