Localization of androgen and estrogen receptors in adult male mouse reproductive tract.
Localization of androgen and estrogen receptors in adult male mouse reproductive tract.
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DOI：
10.1002/j.1939-4640.2002.tb02345.x
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发表时间：
2002-11
影响因子：
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通讯作者：
Qing Zhou;Rong Nie;G. Prins;P. Saunders;B. Katzenellenbogen;R. Hess
中科院分区：
文献类型：
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作者：
Qing Zhou;Rong Nie;G. Prins;P. Saunders;B. Katzenellenbogen;R. Hess
无论是在物种内部还是物种间，关于雄性生殖道中核类固醇受体定位的报道都有相当大的差异。本研究用免疫组织化学方法观察了雄激素受体(AR)和雌激素受体ERα和ERβ在成年雄性小鼠生殖道中的分布，包括睾丸、传出小管、附睾体起始段、附睾头、附睾体和附睾尾以及输精管。免疫印迹和抗体竞争实验证实了抗体的特异性。在睾丸中，AR在间质细胞、支持细胞和大部分小管周细胞中表达，但在生殖细胞中不表达；支持细胞在VI-VI期表达较强，ERα在间质细胞和部分小管周细胞中表达，ERβ在间质细胞、部分小管周细胞、除精子细胞和减数分裂精母细胞外的所有支持细胞和生殖细胞中表达。在传出小管中，AR在纤毛和非纤毛上皮细胞及间质细胞中呈强阳性表达，ERα在纤毛和非纤毛上皮细胞中呈强阳性表达，间质细胞呈阴性表达，ERβ在纤毛和非纤毛上皮细胞及间质细胞中呈强阳性表达。在附睾部，AR在所有上皮细胞中呈强阳性表达(不在上皮内淋巴细胞中)，ERα在起始段的顶端细胞、狭窄细胞和部分基底细胞中呈强阳性表达，在附睾头、头主细胞和尾端远端透明细胞中呈强阳性表达，在起始段间质细胞呈阴性表达，但在从头部到尾部的间质细胞中表达较多；ERβ在附睾头的大部分上皮细胞中呈强阳性表达，但间质细胞表达不一致。在输精管中，AR在主干细胞弱表达或不表达，但在基底细胞呈中等染色，间质中的平滑肌细胞呈强阳性表达，ERα在上皮细胞中不表达，但存在于上皮下平滑肌层，ERβ在所有上皮细胞和大多数间质细胞中均有强表达。这项研究表明，雄性小鼠的生殖道与大鼠的生殖道在AR和ER的表达上有很大的不同，在推断哺乳动物物种的核类固醇受体数据时需要谨慎。
There is considerable variation, both within and between species, in reports of nuclear steroid receptor localizations in the male reproductive tract. In this study, androgen receptor (AR) and estrogen receptors ERalpha and ERbeta were visualized by immunohistochemistry in adult male mice reproductive tracts, including testes, efferent ductules; initial segment, caput, corpus, and cauda epididymides; and vas deferens. Antibody specificity was demonstrated by Western blot and antibody competition. In testis, AR was expressed in Leydig cells, Sertoli cells, and most peritubular cells, but not in germ cells; Sertoli cells showed more intense staining in stages VI-VII; ERalpha was present in Leydig and some peritubular cells; ERbeta was in Leydig, some peritubular, all Sertoli and germ cells except in spermatids and meiotic spermatocytes. In efferent ductules, AR was strongly expressed in ciliated and nonciliated epithelial cells and in stromal cells; ERalpha was strongly expressed in ciliated and nonciliated epithelial cells; stromal cells were negative; and ERbeta was strongly expressed in ciliated and nonciliated epithelial cells and also in stromal cells. In epididymis, AR was strongly expressed in all epithelial cells (not in intraepithelial lymphocytes); ERalpha was strongly expressed in apical, narrow, and some basal cells of the initial segment, and in caput, principal cells of the caput, clear cells of the distal caput through cauda; stromal cells were negative in the initial segment, but more stromal cells were stained from caput to cauda; ERbeta was strongly expressed in most of epithelial cells of the epididymis, but stromal cells were inconsistently stained. In vas deferens, AR was weakly expressed or absent in principal cells but moderately stained in basal cells, smooth muscle cells of stroma were stained intensely, ERalpha was absent in epithelial cells but present in a subepithelial smooth muscle layer, and ERbeta was strongly expressed in all epithelial cells and most stromal cells. This study demonstrates that the reproductive tracts of male mice differ considerably from those of rats in expression of ARs and ERs and that caution is needed when extrapolating nuclear steroid receptor data across mammalian species.