Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications.
Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications.
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DOI：
10.5101/nbe.v7i3.p92-101
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发表时间：
2015
影响因子：
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通讯作者：
B. Abel;Babatunde Odukoya;Muzaffer Mohammed;K. Aslan
中科院分区：
文献类型：
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作者：
B. Abel;Babatunde Odukoya;Muzaffer Mohammed;K. Aslan
我们报道了在生物检测中利用等离子体增强辣根过氧化物酶(HRP)的化学发光响应。等离子体表面由沉积在玻片上的(I)银岛膜(SIF)和(Ii)金属薄膜(银、金、铜和镍，1 nm厚)组成。基于亲和素修饰的HRP与生物素化聚乙二醇胺的单层相互作用的模型生物测定，评估了等离子体表面增强HRP化学发光响应的能力。在模型生物检测中，HRP的化学发光响应比对照样品(即没有等离子体纳米粒子的玻片)增加了~3.7倍，其中高负载的SIF的化学发光响应增强最大。这些发现使我们能够证明使用SIF(高负载)来检测与生物相关的目标蛋白(胶质纤维酸性蛋白或GFAP)，其中对GFAP的标准生物测定的化学发光响应比玻璃片上的生物测定高达~50%。
We report the enhancement of chemiluminescence response of horseradish peroxidase (HRP) in bioassays by plasmonic surfaces, which are comprised of (i) silver island films (SIFs) and (ii) metal thin films (silver, gold, copper, and nickel, 1 nm thick) deposited onto glass slides. A model bioassay, based on the interactions of avidin-modified HRP with a monolayer of biotinylated poly(ethylene-glycol)-amine, was employed to evaluate the ability of plasmonic surfaces to enhance chemiluminescence response of HRP. Chemiluminescence response of HRP in model bioassays were increased up to ~3.7-fold as compared to the control samples (i.e. glass slides without plasmonic nanoparticles), where the largest enhancement of the chemiluminescence response was observed on SIFs with high loading. These findings allowed us to demonstrate the use of SIFs (high loading) for the detection of a biologically relevant target protein (glial fibrillary acidic protein or GFAP), where the chemiluminescence response of the standard bioassay for GFAP was enhanced up to ~50% as compared to bioassay on glass slides.