Simple and sensitive determination of free and total morphine in human liver and kidney using gas chromatography-mass spectrometry
Simple and sensitive determination of free and total morphine in human liver and kidney using gas chromatography-mass spectrometry
复制标题
DOI：
10.1016/j.jchromb.2005.10.049
复制
发表时间：
2006-01-18
影响因子：
3
通讯作者：
Ikeda, N
中科院分区：
文献类型：
--
作者：
Kudo, K;Ishida, T;Ikeda, N
建立了一种可靠、简便、灵敏的气相色谱-质谱联用（GC-MS）测定人体肝、肾组织中游离吗啡和总吗啡的方法。使用Extremut（R）NT柱用内标二氢可待因提取游离吗啡或通过酸水解从0.2g组织样品中获得的总吗啡，然后进行三甲基甲硅烷基化。将衍生化的提取物进行EI-SIM模式的GC-MS分析。在0.005 ~ 5 μ g/g浓度范围内，肝、肾样品中吗啡的线性关系良好。吗啡的检测下限为0.005 μ g/g。对一名因在医院误服吗啡类化合物而导致吗啡中毒死亡的尸检病人的肝、肾组织中游离吗啡和总吗啡含量进行了测定，结果表明该方法是成功的。(c)2005 Elsevier B. V.保留所有权利。
We developed a reliable, simple and sensitive method to determine free and total morphine in human liver and kidney, using gas chromatography-mass spectrometry (GC-MS). Free morphine or total morphine obtained by acid hydrolysis from 0.2 g tissue sample was extracted using an Extrelut((R)) NT column with an internal standard, dihydrocodeine, followed by trimethylsilylation. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of morphine in both liver and kidney samples were linear in the concentration range from 0.005 to 5 mu g/g. The lower limits of detection of morphine were 0.005 mu g/g. This method proved successful when we determined free and total morphine in liver and kidney obtained from an autopsied man who was mis-ingested morphine compound in the hospital, which resulted in the cause of death being morphine intoxication. (c) 2005 Elsevier B.V. All rights reserved.