Stored red blood cell susceptibility to in vitro transfusion-associated stress conditions is higher after longer storage and increased by storage in saline-adenine-glucose-mannitol compared to AS-1.
Stored red blood cell susceptibility to in vitro transfusion-associated stress conditions is higher after longer storage and increased by storage in saline-adenine-glucose-mannitol compared to AS-1.
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DOI：
10.1111/trf.13138
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发表时间：
2015-09
期刊：
影响因子：
2.9
通讯作者：
Sparrow RL
中科院分区：
文献类型：
--
作者：
Mittag D;Sran A;Chan KS;Boland MP;Bandala-Sanchez E;Huet O;Xu W;Sparrow RL
红细胞在储存过程中引起的生化变化可能会损害其在输血后的功能。输血相关的应激可进一步放大储存损伤效应，包括增加红细胞膜上磷脂酰丝氨酸（PS）的暴露，微粒（MP）的释放和内皮细胞（ECs）的粘附。研究了红细胞体外应激敏感性与贮藏时间和添加溶液的关系。将白细胞减少的全血捐献（n=18）配对、混合并重新分裂，然后分离红细胞保存在SAGM或AS-1溶液中。分别于3、21、35天后取样。对于氧化应激处理，红细胞暴露于0.5mM叔丁基过氧化氢。用含炎症介质的血浆在37°C下过夜培养模拟输血相关应激。流式细胞术检测ps暴露和MPs，并在流动条件下检测对EC的粘附。采用含PS脂质囊泡阻断法检测PS粘附特异性。氧化应激诱导保存35天的红细胞中ps暴露和ECs粘附显著高于保存3天的红细胞（p<0.04）。含ps的囊泡阻断了红细胞- ec的粘附。加血浆或不加血浆培养过夜后，ps暴露和ec粘附均显著升高（p<0.05）。随着红细胞储存时间的延长和红细胞血浆培养后，MP数增加。培养条件影响第35天红细胞的MP数。应激处理后，储存在SAGM中的红细胞的ps暴露量显著高于AS-1红细胞（p<0.02）。储存35天显著增加了红细胞对氧化和体外输血相关应激的敏感性，与AS-1相比，储存在SAGM中的红细胞的敏感性更高。
Biochemical changes induced in red blood cells (RBCs) during storage may impair their function upon transfusion. Transfusion-associated stresses may further amplify storage lesion effects including increased phosphatidylserine (PS)-exposure at the RBC membrane, microparticle (MP)-release and adhesion to endothelial cells (ECs). RBC stress susceptibility in vitro was investigated in relation to storage time and additive solution. Leukocyte-reduced whole blood donations (n=18) were paired, mixed and re-split before separating the RBCs for storage in SAGM or AS-1 solutions. Samples were taken after 3, 21 or 35 days. For oxidative stress treatment RBCs were exposed to 0.5mM tert-butylhydroperoxide. Transfusion-associated stress was simulated by overnight culture at 37 °C with plasma containing inflammatory mediators. PS-exposure and MPs were measured by flow cytometry and adhesion to EC was tested under flow conditions. PS specificity of adhesion was tested by blocking with PS-containing lipid vesicles. Oxidative stress induced significantly higher PS-exposure and adhesion to ECs in RBCs stored for 35 days compared to 3 days (p<0.04). PS-containing vesicles blocked RBC-EC adhesion. After overnight culture with or without plasma, PS-exposure and EC-adhesion were significantly increased (p<0.05). MP numbers increased with longer RBC storage and after RBC culture with plasma. Culture conditions influenced MP numbers from day 35 RBCs. RBCs stored in SAGM had significantly higher PS-exposure after stress treatment than AS-1 RBCs (p<0.02). Storage for 35 days significantly increased RBC susceptibility to oxidative and in vitro transfusion-associated stresses and was higher for RBCs stored in SAGM compared to AS-1.