Variation in MHC expression between undifferentiated mouse ES cells and ES cell-derived insulin-producing cell clusters.
Variation in MHC expression between undifferentiated mouse ES cells and ES cell-derived insulin-producing cell clusters.
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DOI：
10.1097/tp.0b013e3181a19421
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发表时间：
2009-05-15
期刊：
影响因子：
6.2
通讯作者：
Wood KJ
中科院分区：
文献类型：
--
作者：
Boyd AS;Wood KJ
胚胎干细胞（ES）的后代可能最终用于移植中替代受损组织，但其免疫原性仍不明确。主要组织相容性复合体（MHC）是移植免疫原性的决定因素。在此，我们展示了小鼠胚胎干细胞和胚胎干细胞衍生的胰岛素产生细胞簇（ipcc）之间MHC表达的差异，包括ipcc中MHC I类的表达相对较高，干扰素-γ (IFN-γ)刺激后ipcc中MHC I类的诱导更快、更显著。暴露于IFN-γ后，在ipcc上诱导MHC II类，而不是在ES细胞上。同种或异体ipc移植在移植后3天内不足以触发MHC I类上调。这些数据突出了胚胎干细胞和完全分化的胚胎干细胞衍生组织之间MHC表达的差异，并表明胚胎干细胞的后代在移植后可能容易发生排斥反应。
The progeny of embryonic stem (ES) cells may eventually be used to replace damaged tissues in transplantation, yet their immunogenicity remains ill-defined. The major histocompatibility complex (MHC) is a determinant of immunogenicity in transplantation. Herein, we show differences in MHC expression between mouse ES cells and ES cell derived insulin producing cell clusters (IPCCs), including a relatively higher expression of MHC Class I in IPCCs and a faster, more dramatic induction of MHC Class I in IPCCs following challenge with interferon-γ (IFN-γ). MHC Class II was induced on IPCCs, but not ES cells, after exposure to IFN-γ. Transplantation of syngeneic or allogeneic IPCCs was insufficient to trigger up-regulation of MHC class I within three days after transplantation. These data highlight differences in MHC expression between ES cells and a fully differentiated ES cell derived tissue and suggest how the progeny of ES cells may be susceptible to rejection after transplantation.