Immunohistochemical Analysis Using Monoclonal Antibody PMab-269 Against Steller Sea Lion Podoplanin
Immunohistochemical Analysis Using Monoclonal Antibody PMab-269 Against Steller Sea Lion Podoplanin
复制标题
使用针对 Steller Sea Lion Podoplanin 的单克隆抗体 PMab-269 进行免疫组织化学分析
DOI：
10.1089/mab.2021.0055
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发表时间：
2022
影响因子：
--
通讯作者：
Yukinari Kato
中科院分区：
文献类型：
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作者：
Guanjie Li;Hiroyuki Suzuki;Junko Takei;Masaki Saito;Nohara Goto;Kazuyuki Uchida;Takayuki Nakagawa;Hiroyuki Harada;Tomohiro Tanaka;Teizo Asano;Mika K. Kaneko;Yukinari Kato
免疫组织化学分析需要针对I型肺泡细胞标志物足普兰(PDPN)的单抗(MAbs)。抗PDPN单抗可用于许多物种，包括人、鼠、鼠、兔、狗、猫、牛、猪、塔斯马尼亚魔鬼、羊驼、老虎、鲸鱼、山羊、马、熊、绵羊和加州海狮PDPN。然而，目前还没有针对Steller海狮PDPN(StePDPN)的抗体。免疫组织化学分析表明，抗加利福尼亚海狮PDPN单抗(PMab-269)可与Steller海狮肺、肾小球和肾包膜的I型细胞以及结肠的淋巴管内皮细胞反应，表明PMab-269可用于检测StePDPN。
Monoclonal antibodies (mAbs) that specifically target podoplanin (PDPN), a marker for type I alveolar cells, are required for immunohistochemical analyses. Anti-PDPN mAbs are available for many species, including human, mouse, rat, rabbit, dog, cat, bovine, pig, Tasmanian devil, alpaca, tiger, whale, goat, horse, bear, sheep, and California sea lion PDPNs. However, no anti-Steller sea lion PDPN (stePDPN) antibody has been developed. Immunohistochemical analysis showed that an anti-California sea lion PDPN mAb (PMab-269) reacted with type I alveolar cells from the Steller sea lion lung, renal glomeruli and Bowman's capsules from kidney, and lymphatic endothelial cells from the colon, indicating that PMab-269 is useful for detecting stePDPN.