Hormonal regulation and properties of a new group of basic hemolymph proteins expressed during insect metamorphosis.
Hormonal regulation and properties of a new group of basic hemolymph proteins expressed during insect metamorphosis.
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DOI：
10.1016/s0021-9258(18)54072-2
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发表时间：
1993-01
期刊：
影响因子：
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通讯作者：
Grace Jones;M. Mańczak;M. Horn
中科院分区：
文献类型：
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作者：
Grace Jones;M. Mańczak;M. Horn
两个丰富的碱性蛋白质，arylphorin，在变态的粉纹夜蛾ni的表达分离，其cDNA克隆，并调节其表达的评估。根据编码的蛋白质序列的标准，序列共同起始和共同终止的位置，氨基酸组成，非性别特异性，和免疫分析，相关的但不同的碱性蛋白质不对应于任何先前建立的昆虫蛋白质组，其中每一个可以区分的基础上，这样的标准，虽然它们似乎是在节肢动物血蓝蛋白超家族。碱性蛋白质可以在天然等电聚焦条件下分离，表明它们在这种条件下不优先形成异聚复合物。虽然碱性蛋白和共表达的酸性蛋白的转录物（Jones，G.，布朗，N.，Manczak，M.，Hiremath，S.，和Kafatos，F.（1990）J.Biol.Chem.265，8596-8602）在蜕皮类固醇驱动的变质定型的前一天出现在两性中，并在接下来的24小时内以高丰度持续，芳基转运蛋白mRNA提前1天出现，并在变质定型后立即下降。动态抑制的外观和丰富的成绩单，以维持高保幼激素滴度是不同的酸性与两种碱性蛋白质。对保幼激素反应的另一个调节差异表现为两种碱性蛋白质的mRNA的可翻译性降低，但酸性蛋白质的可翻译性不降低。相反，arylphorin mRNA的丰度和可翻译性对高保幼激素水平不敏感。调节中的进一步差异表现为在其转录物消失后血淋巴中保持高水平的arylphorin和酸性蛋白，而碱性蛋白则从血淋巴中消失并持续存在于脂肪体中。这些结果建立了至少三个定性不同的影响保幼激素对这些代表的血蓝蛋白超家族中的三个组的转录丰度，也建立mRNA的可翻译性作为另一个差异水平的调节。考虑到进化相关蛋白调节机制的差异，T。在研究幼虫组织变态转化过程中激素对基因表达的调节机制方面，Ni应被证明是一种有价值的工具。
Two abundant basic proteins, and arylphorin, that are expressed during metamorphosis of Trichoplusia ni were isolated, their cDNAs cloned, and regulation of their expression assessed. According to the criteria of encoded protein sequence, positions of sequence coinitiation and cotermination, amino acid compositions, nonsex-specificity, and immunological analyses, the related yet distinct basic proteins do not correspond to any previously established group of insect proteins, each of which can be distinguished on the basis of such criteria, although they appear to be within the arthropod hemocyanin superfamily. The basic proteins can be separated under native isoelectric focusing conditions, indicating that they do not preferentially form heteromeric complexes under such conditions. While the transcripts for both basic proteins and a coexpressed acidic protein (Jones, G., Brown, N., Manczak, M., Hiremath, S., and Kafatos, F. (1990) J. Biol. Chem. 265, 8596-8602) appeared in both sexes on the day prior to ecdysteroid-driven metamorphic commitment, and persisted at high abundance for the next 24 h, the arylphorin mRNA appeared 1 day earlier and declined immediately after metamorphic commitment. The dynamics of suppression of the appearance and abundance of transcripts in response to maintenance of a high juvenile hormone titer was different for the acidic versus the two basic proteins. Another regulatory difference in response to juvenile hormone was shown by a decreased translatability of the mRNAs for the two basic proteins, but not for the acidic protein. In contrast, the abundance and translatability of arylphorin mRNA was insensitive to a high juvenile hormone level. A further difference in regulation was shown by the maintenance of a high level of both arylphorin and the acidic protein in the hemolymph after disappearance of their transcripts, while the basic proteins instead disappeared from the hemolymph and persisted in the fat body. These results establish at least three qualitatively different effects of juvenile hormone on the abundance of transcripts for these representatives of three groups in the hemocyanin superfamily, and also establish mRNA translatability as another differential level of regulation among them. Given these differences in mechanisms of regulation of evolutionarily related proteins, this system of metamorphosis-associated proteins in T. ni should prove to be a valuable tool in studies of mechanisms of hormonal regulation of gene expression during the metamorphic transformation of larval tissues.