Efficacy of antimicrobial photodynamic therapy with Rose Bengal and blue light against cariogenic bacteria
Efficacy of antimicrobial photodynamic therapy with Rose Bengal and blue light against cariogenic bacteria
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DOI：
10.1016/j.archoralbio.2020.105024
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发表时间：
2021-02-01
影响因子：
3
通讯作者：
Shibuya, Yasuyuki
中科院分区：
文献类型：
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作者：
Hirose, Marina;Yoshida, Yasuo;Shibuya, Yasuyuki
目的：我们评估了使用虎红和蓝光LED的抗微生物光动力疗法（a-PDT）对引发和促进龋齿的细菌的影响。设计：在使用虎红和蓝光LED的PDT治疗后，对变形链球菌、远缘链球菌、血链球菌和唾液乳杆菌在无菌和生物膜条件下的菌落形成单位进行计数。此外，来自10名志愿者的唾液和牙菌斑的致龋细菌用于评估a-PDT治疗。结果：我们发现，使用虎红的PDT在> 10 μ g/mL时对口腔革兰氏阳性S. mutans，S. sobrinus，S. sanguinis和L.唾液分泌和生物膜条件下。对形成含有水不溶性葡聚糖的生物膜的致龋细菌也观察到了这种效果，通过这种生物膜细菌牢固地附着在牙齿表面。此外，a-PDT导致唾液和牙菌斑中的致龋菌显着减少。结论：PDT可能是一种有用的方法，用于控制牙科手术中的龋齿。
Objective: We evaluated the effect of antimicrobial photodynamic therapy (a-PDT) with Rose Bengal and blue light LED on bacteria that initiate and promote dental caries.Design: Colony forming units of Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, and Lactobacillus salivarius under planktonic and biofilm conditions were counted after a PDT treatment using Rose Bengal and blue light LED. In addition, cariogenic bacteria from saliva and dental plaques from ten volunteers were used for evaluation of a-PDT treatment.Results: We found that a PDT using Rose Bengal at > 10 mu g/mL had antimicrobial effects on oral Gram-positive S. mutans, S. sobrinus, S. sanguinis, and L. salivarius under both planktonic and biofilm conditions. The effect was also observed for cariogenic bacteria that formed biofilms containing water-insoluble glucans, through which the bacteria are firmly attached to the tooth surface. Moreover, a-PDT led to a marked reduction in cariogenic bacteria in saliva and dental plaques.Conclusion: a PDT could be a useful approach for controlling dental caries in dental surgery.