Dek35 Encodes a PPR Protein that Affects cis-Splicing of Mitochondrial nad4 Intron 1 and Seed Development in Maize
Dek35 Encodes a PPR Protein that Affects cis-Splicing of Mitochondrial nad4 Intron 1 and Seed Development in Maize
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DOI：
10.1016/j.molp.2016.08.008
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发表时间：
2017-03-06
期刊：
影响因子：
27.5
通讯作者：
Song, Rentao
中科院分区：
文献类型：
--
作者：
Chen, Xinze;Feng, Fan;Song, Rentao
在高等植物中，细胞器编码mRNA的剪接涉及到与核烯编码蛋白的复杂合作。五肽重复（PPR）蛋白参与了这些rna -蛋白相互作用。本研究对玉米缺陷粒35 （Dek35）进行了克隆和功能鉴定。dek35-ref突变体是一种具有发育缺陷的致命种子突变体。Dek35通过Mutator标签分离克隆，并通过另外4个独立突变等位基因进一步证实。Dek35编码一种靶向线粒体的p型PPR蛋白。dek35突变导致dek35蛋白积累显著减少，线粒体nad4内含子1剪接效率降低。对dek35未成熟种子线粒体复合体的分析表明，复合体I组装和NADH脱氢酶活性严重不足。dek35胚乳的转录组分析显示，参与替代呼吸途径的基因和与线粒体功能和活性相关的广泛差异表达基因的表达增强。总之，这些结果表明Dek35编码一种PPR蛋白，该蛋白影响线粒体nad4内含子1的顺式剪接，并且是线粒体功能和种子发育所必需的。
In higher plants, the splicing of organelle-encoded mRNA involves a complex collaboration with nuclearencoded proteins. Pentatricopeptide repeat (PPR) proteins have been implicated in these RNA-protein interactions. In this study, we performed the cloning and functional characterization of maize Defective kernel 35 ( Dek35). The dek35-ref mutant is a lethal-seed mutant with developmental deficiency. Dek35 was cloned through Mutator tag isolation and further confirmed by four additional independent mutant alleles. Dek35 encodes an P-type PPR protein that targets the mitochondria. The dek35 mutation causes significant reduction in the accumulation of DEK35 proteins and reduced splicing efficiency of mitochondrial nad4 intron 1. Analysis of mitochondrial complex in dek35 immature seeds indicated severe deficiency in the complex I assembly and NADH dehydrogenase activity. Transcriptome analysis of dek35 endosperm revealed enhanced expression of genes involved in the alternative respiratory pathway and extensive differentially expressed genes related to mitochondrial function and activity. Collectively, these results indicate that Dek35 encodes an PPR protein that affects the cis-splicing of mitochondrial nad4 intron 1 and is required for mitochondrial function and seed development.