Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering
Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering
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DOI：
10.1002/term.176
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发表时间：
2009-07-01
影响因子：
3.3
通讯作者：
Detamore, Michael S.
中科院分区：
文献类型：
--
作者：
Wang, Limin;Singh, Milind;Detamore, Michael S.
人脐带间充质基质细胞（Human umbilical cord mesenchymal stromal cells，hUCMSCs）最近显示出在所有三个胚胎胚层中分化成多个细胞谱系的能力。hUCMSCs在单层培养中的成骨分化已有报道，而在三维生物材料中的分化尚未报道用于组织工程应用。因此，本研究的目的是评估使用hUCMSCs用于骨组织工程的可行性。将hUCMSC在成骨培养基（OM）中的聚（L-乳酸）（PLLA）支架中培养3周，之后将支架暴露于几种不同的培养基，包括OM、矿化培养基（MM）和具有10或100 ng/ml胰岛素样生长因子（IGF）-1的MM。通过Runx 2和OCN的上调、钙定量和骨组织学证实成骨分化。从OM切换到MM促进胶原蛋白合成和每个细胞的钙含量，而继续在OM中保留更多的细胞在构建体中，并促进更高的成骨基因表达。IGF-1对MM细胞增殖、分化及基质合成无影响。总之，hUCMSCs在骨组织工程中显示出巨大的潜力，并且在整个期间在OM中培养有利于这些细胞的成骨分化。版权所有（C）2009约翰威利父子有限公司
Human umbilical cord mesenchymal stromal cells (hUCMSCs) have recently shown the capacity to differentiate into multiple cell lineages in all three embryonic germ layers. The osteogenic differentiation of hUCMSCs in monolayer culture has been reported, while the differentiation in three-dimensional biomaterials has not yet been reported for tissue-engineering applications. Thus, the aim of this study was to evaluate the feasibility of using hUCMSCs for bone tissue engineering. hUCMSCs were cultured in poly(L-lactic acid) (PLLA) scaffolds in osteogenic medium (OM) for 3 weeks, after which the scaffolds were exposed to several different media, including the OM, a mineralization medium (MM) and the MM with either 10 or 100 ng/ml insulin-like growth factor (IGF)-1. The osteogenic differentiation was confirmed by the up-regulation of Runx2 and OCN, calcium quantification and bone histology. Switching from the OM to the MM promoted collagen synthesis and calcium content per cell, while continuing in the OM retained more cells in the constructs and promoted higher osteogenic gene expression. The addition of IGF-1 into the MM had no effect on cell proliferation, differentiation and matrix synthesis. In conclusion, hUCMSCs show significant potential for bone tissue engineering and culturing in the OM throughout the entire period is beneficial for osteogenic differentiation of these cells. Copyright (C) 2009 John Wiley & Sons, Ltd.