In vitro cytotoxicitiy of silica nanoparticles at high concentrations strongly depends on the metabolic activity type of the cell line
In vitro cytotoxicitiy of silica nanoparticles at high concentrations strongly depends on the metabolic activity type of the cell line
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DOI：
10.1021/es062347t
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发表时间：
2007-03-15
影响因子：
11.4
通讯作者：
Kong, Zwe-Ling
中科院分区：
文献类型：
--
作者：
Chang, Jenq-Sheng;Chang, Ke Liang B.;Kong, Zwe-Ling
无定形二氧化硅越来越多地用于诊断和生物医学研究，因为它易于生产和相对较低的成本。它通常被认为是安全的，并已被批准用作食品或动物饲料成分。最近的文献表明，无定形二氧化硅在高剂量下可能存在毒性问题。在预期潜在的人体暴露于二氧化硅，它是可取的，以检查其对不同器官的细胞的毒性。因此，我们研究了几种正常的成纤维细胞和肿瘤细胞对不同剂量的无定形二氧化硅或二氧化硅和壳聚糖的复合纳米颗粒的反应。细胞增殖试验表明，二氧化硅纳米颗粒在低剂量下是无毒的，但在高剂量下细胞活力降低。乳酸脱氢酶（LDH）测定表明，高剂量的二氧化硅诱导细胞膜损伤。这两种试验都表明，具有长倍增时间的成纤维细胞比具有短倍增时间的肿瘤细胞更容易受到二氧化硅暴露诱导的损伤。相比之下，二氧化硅-壳聚糖复合纳米颗粒诱导较少的细胞增殖抑制和较少的膜损伤。这项研究表明，二氧化硅对人体细胞的细胞毒性强烈依赖于它们的代谢活动，但它可以显着降低合成二氧化硅与壳聚糖。
Amorphous silica is increasingly used in diagnostic and biomedical research because of its ease of production and relatively low cost. It is generally regarded as safe and has been approved for use as a food or animal feed ingredient. Recent literature reveals that amorphous silica may present toxicity concerns at high doses. In anticipation of potential human exposure to silica, it is advisable to examine its toxicity to cells of different organs. Consequently, we investigated the response of several normal fibroblast and tumor cells to varying doses of amorphous silica or composite nanoparticles of silica and chitosan. A cell proliferation assay indicates that silica nanoparticles are nontoxic at low dosages but that cell viability decreases at high dosages. A lactate dehydrogenase (LDH) assay indicates that high dosages of silica induce cell membrane damage. Both assays reveal that fibroblast cells with long doubling times are more susceptible to injury induced by silica exposure than tumor cells with short doubling times. In contrast, silica-chitosan composite nanoparticles induce less inhibition in cell proliferation and less membrane damage. This study suggests that the cytotoxicity of silica to human cells depends strongly on their metabolic activities but that it could be significantly reduced by synthesizing silica with chitosan.