THE FORMATION OF DAPTOMYCIN BY SUPPLYING DECANOIC ACID TO STREPTOMYCES-ROSEOSPORUS CULTURES PRODUCING THE ANTIBIOTIC COMPLEX A21978C
THE FORMATION OF DAPTOMYCIN BY SUPPLYING DECANOIC ACID TO STREPTOMYCES-ROSEOSPORUS CULTURES PRODUCING THE ANTIBIOTIC COMPLEX A21978C
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DOI：
10.1016/0168-1656(88)90040-5
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发表时间：
1988-04-01
影响因子：
4.1
通讯作者：
TIETZ, AJ
中科院分区：
文献类型：
--
作者：
HUBER, FM;PIEPER, RL;TIETZ, AJ
抗生素物质A21978C是一种具有共同环状多肽核和不同脂肪酸侧链的复合物。达托霉素是一种半合成的抗菌物质，由A21978C复合物通过一个非常复杂的化学过程衍生而来。为了获得达托霉素，我们首先从玫瑰孢链霉菌的培养滤液中通过多种树脂分离得到A21978C配合物。然后将产生的物质“阻断”并添加到放线素平面培养中进行去酰化。受保护的A21978C细胞核随后通过与母体复合体相同的程序分离，并与所需的脂肪酸（癸酸）再酰基化。酰基化的化合物被分解得到达托霉素。本报告描述了为建立一种战略而进行的实验，该战略是向玫瑰孢杆菌培养物提供一种毒性很强的前体，从而生物合成生产达托霉素。
Antibiotic substance A21978C is a complex of compounds having a common cyclic polypeptide nucleus and different fatty acid sidechains. Daptomycin is a semi-synthetic antimicrobial substance derived from the A21978C complex by a very elaborate chemical process. To obtain the daptomycin, the A21978C complex was first isolated from culture filtrates of Streptomyces roseosporus by several resin procedures. The resulting material was then ''blocked'' and added to an Actinoplanes culture for deacylation. The protected A21978C nucleus was subsequently isolated by the same procedure as the parent complex and reacylated with the desired fatty acid (decanoic acid). The acylated compound was deblocked to yield daptomycin. This report describes the experimentation undertaken to establish a strategy to supply a very toxic precursor to cultures of S. roseosporus and, thereby, produce daptomycin biosynthetically.