Design of Biomimetic Cell-Interactive Substrates Using Hyaluronic Acid Hydrogels with Tunable Mechanical Properties
Design of Biomimetic Cell-Interactive Substrates Using Hyaluronic Acid Hydrogels with Tunable Mechanical Properties
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DOI：
10.1021/bm300324m
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发表时间：
2012-06-01
影响因子：
6.2
通讯作者：
Auzely-Velty, Rachel
中科院分区：
文献类型：
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作者：
Hachet, Emilie;Van Den Berghe, Helene;Auzely-Velty, Rachel
透明质酸（HA）是一种存在于生物组织中的天然多糖，具有很好的合成细胞外基质类似物的潜力。在这项工作中，我们建立了一个简单可靠的方法来制备透明质酸为基础的水凝胶与控制刚度和细胞识别性能作为细胞相互作用的基板。这种方法依赖于一种新的甲基丙烯酸酯改性的HA大分子聚合物（HA-MA）的合成程序，并在光流变学允许真实的时间监测凝胶化过程中光聚合。我们以这种方式显示了获得凝胶的能力，所述凝胶包括生理相关弹性模量的范围，同时仍然保持HA通过特定细胞表面受体的识别特性。这些水凝胶由具有一定程度甲基丙烯酸酯化的HA大分子单体制备
Hyaluronic acid (HA) is a natural polysaccharide abundant in biological tissues with excellent potential for constructing synthetic extracellular matrix analogues. In this work, we established a simple and dependable approach to prepare hyaluronic acid-based hydrogels with controlled stiffness and cell recognition properties for use as cell-interactive substrates. This approach relied on a new procedure for the synthesis of methacrylate-modified HA macromers (HA-MA) and, on photorheometry allowing real time monitoring of gelation during photopolymerization. We showed in this way the ability to obtain gels that encompass the range of physiologically relevant elastic moduli while still maintaining the recognition properties of HA by specific cell surface receptors. These hydrogels were prepared from HA macromers having a degree of methacrylation