An Uncharacterized Major Facilitator Superfamily Transporter From Planococcus maritimus Exhibits Dual Functions as a Na(+)(Li(+), K(+))/H(+) Antiporter and a Multidrug Efflux Pump.
An Uncharacterized Major Facilitator Superfamily Transporter From Planococcus maritimus Exhibits Dual Functions as a Na(+)(Li(+), K(+))/H(+) Antiporter and a Multidrug Efflux Pump.
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来自海平球菌的一种未表征的主要促进剂超家族转运蛋白具有 Na (Li , K )/H 反向转运蛋白和多药物外排泵的双重功能
DOI：
10.3389/fmicb.2018.01601
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发表时间：
2018
影响因子：
5.2
通讯作者：
Jiang J
中科院分区：
文献类型：
--
作者：
Abdel-Motaal H;Meng L;Zhang Z;Abdelazez AH;Shao L;Xu T;Meng F;Abozaed S;Zhang R;Jiang J
在主要促进因子超家族（MFS）中，多达27个未知的主要促进因子家族和60个已被充分表征的家族中的许多成员由于与已表征的MFS成员没有或非常低的序列同源性而在功能上未知。在这里，我们首次报道了一种功能未知的MFS转运蛋白的特征，该转运蛋白被命名为MdrP，其加入版本为MdrP。ANU18183.1来自轻微嗜盐的Planococcus maritimus DS 17275T。在Na+/H+反转运基因筛选过程中，我们首次发现MdrP具有Na+(Li+, K+)/H+反转运活性，并提出它可能代表了一类新的Na+(Li+, K+)/H+反转运基因。然而，我们推测MdrP可能具有额外的蛋白质功能。药物/H+反转运体（DHA）家族成员的特征Motif A的存在和系统发育分析表明，MdrP也可能具有药物外排泵的功能，这一点通过最低抑制浓度试验和药物外排活性测定得到证实。综上所述，这种新型的MFS转运蛋白具有Na+(Li+, K+)/H+反向转运蛋白和多药物外排泵的双重功能，这不仅有助于对未表征的MFS成员特别是DHA1家族成员的功能预测，而且有助于拓宽对Na+/H+反向转运蛋白的认识。
Within major facilitator superfamily (MFS), up to 27 unknown major facilitator families and many members of 60 well-characterized families have been functionally unknown as yet, due to their sharing no or significantly low sequence identity with characterized MFS members. Here we present the first report on the characterization of one functionally unknown MFS transporter designated MdrP with the accession version No. ANU18183.1 from the slight halophile Planococcus maritimus DS 17275T. During the screening of Na+/H+ antiporter genes, we found at first that MdrP exhibits Na+(Li+, K+)/H+ antiport activity, and propose that it should represent a novel class of Na+(Li+, K+)/H+ antiporters. However, we speculate that MdrP may possess an additional protein function. The existence of the signature Motif A of drug/H+antiporter (DHA) family members and phylogenetic analysis suggest that MdrP may also function as a drug efflux pump, which was established by minimum inhibitory concentration tests and drug efflux activity assays. Taken together, this novel MFS transporter exhibits dual functions as a Na+(Li+, K+)/H+ antiporter and a multidrug efflux pump, which will be very helpful to not only positively contribute to the function prediction of uncharacterized MFS members especially DHA1 family ones, but also broaden the knowledge of Na+/H+ antiporters.
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影响因子：
2.8
作者：
Bannam, TL;Johanesen, PA;Rood, JI
通讯作者：
Rood, JI
假定的配对小多药耐药家族蛋白 PsmrAB（YvdSR 的同源物）实际上充当新型双组分 Na /H 反向转运蛋白
影响因子：
2.1
作者：
Jiang, Juquan;Wang, Lei;Yang, Lifu
通讯作者：
Yang, Lifu
影响因子：
3.2
作者：
Edgar, R;Bibi, E
通讯作者：
Bibi, E
影响因子：
3.6
作者：
Krulwich TA;Hicks DB;Ito M
通讯作者：
Ito M
影响因子：
3.2
作者：
Herz, K;Vimont, S;Berche, P
通讯作者：
Berche, P