Hydrophilic Interaction Chromatography
Hydrophilic Interaction Chromatography
复制标题
DOI：
10.1134/s1061934819050058
复制
发表时间：
2019-05
影响因子：
1.1
通讯作者：
L. Kartsova;E. Bessonova;V. Somova
中科院分区：
文献类型：
--
作者：
L. Kartsova;E. Bessonova;V. Somova
亲水作用液相色谱（HILIC）是近年来发展较为活跃的一种色谱技术，它包括分析物的分配、吸附、离子交换和排阻等。本文综述了该方法的原理、固定相和移动的相的类型、HILIC条件下高极性化合物的保留机理、二维色谱的实现以及该方法的应用领域.关键词：亲水色谱、保留机理、反相高效液相色谱、正相模式、HILIC固定相、两性离子相、高极性分析物
AbstractHydrophilic interaction liquid chromatography (HILIC), which includes the distribution, adsorption, ion exchange, and exclusion of analytes, has gained active development recently. The review covers the principles of the method, types of stationary and mobile phases, mechanisms of retention of highly polar compounds under HILIC conditions, implementation of 2D chromatography, and also fields of application of the method.Keywords: hydrophilic chromatography, retention mechanism, reverse phase HPLC, normal phase mode, HILIC stationary phases, zwitterionic phases, high polar analytes