Export of antigenic peptides from the endoplasmic reticulum intersects with retrograde protein translocation through the Sec61p channel
Export of antigenic peptides from the endoplasmic reticulum intersects with retrograde protein translocation through the Sec61p channel
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DOI：
10.1016/s1074-7613(00)00013-3
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发表时间：
2000-07-01
期刊：
影响因子：
32.4
通讯作者：
Momburg, F
中科院分区：
文献类型：
--
作者：
Koopmann, JO;Albring, J;Momburg, F
抗原肽通过 TAP 肽转运蛋白从胞质溶胶转运到内质网 (ER)，以装载到 MHC I 类分子上。无法结合的肽需要从 ER 中去除。在这里，我们提供了肽输出利用 Sec61p 易位子的证据，如用细菌外毒素阻断该通道所证明的那样。肽输出干扰β(2)-微球蛋白从内质网到胞质溶胶的逆转位，表明蛋白质和寡肽的处理途径相似。肽输出需要 ATP 供应至 ER 腔，但与 ATP 水解无关。
Antigenic peptides are translocated by the TAP peptide transporter from the cytosol into the endoplasmic reticulum (ER) for loading onto MHC class I molecules. Peptides that fail to bind need to be removed from the ER. Here we provide evidence that peptide export utilizes the Sec61p translocon as demonstrated by blocking this channel with bacterial exotoxin. Peptide export interferes with the retrotranslocation of beta(2)-microglobulin from the ER to the cytosol, suggesting similar pathways for the disposal of proteins and oligopeptides. Peptide export requires ATP supply to the ER lumen but is independent of ATP hydrolysis.