Cortical neurogenesis enhanced by chronic perinatal hypoxia
Cortical neurogenesis enhanced by chronic perinatal hypoxia
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DOI：
10.1016/j.expneurol.2005.04.006
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发表时间：
2006-05-01
影响因子：
5.3
通讯作者：
Vaccarino, Flora M.
中科院分区：
文献类型：
--
作者：
Fagel, Devon M.;Ganat, Yosif;Vaccarino, Flora M.
在这里，我们报告说，一个低水平的神经发生发生在大脑皮层的婴儿小鼠大脑和增强慢性围产期缺氧。当小鼠出生后第3天至第11天在低氧环境中饲养时，大约30%的皮质神经元在损伤后丢失;但这种损伤是短暂的。在恢复期间，皮质神经元数量、皮质体积和脑重量的损失均得到逆转。在P 18，缺氧停止后7天，SVZ内星形胶质细胞增殖显著增加，如通过5-溴脱氧尿苷（BrdU）掺入S期细胞所评估的。一个月后，BrdU掺入，40%以上的BrdU阳性细胞被发现在大脑皮质的缺氧饲养相比，常氧对照小鼠。在这些新产生的皮质细胞中，在缺氧和常氧小鼠中，约45%是少突胶质细胞，35%是星形胶质细胞，10%是神经元。然而，与对照组相比，在从缺氧中恢复的小鼠的新皮层中，有两倍的BrdU标记细胞表达神经元标记物。在缺氧饲养和常氧的婴儿/幼年小鼠，可以看到假定的神经母细胞脱离前脑室下区，迁移通过皮质下白色物质，并进入较低的皮质层，5至11天后，他们的最后一次有丝分裂。我们认为，皮质神经发生可能在修复新生儿损伤后的神经元损失中发挥重要作用。(c)2005年爱思唯尔公司All rights reserved.
Here, we report that a low level of neurogenesis occurs in the cerebral cortex of the infant mouse brain and is enhanced by chronic perinatal hypoxia. When mice were reared in a low-oxygen environment from postnatal days 3 to 11, approximately 30% of the cortical neurons were lost after the insult; yet this damage was transient. The loss of cortical neuron number, cortical volume, and brain weight were all reversed during the recovery period. At P 18, 7 days after the cessation of hypoxia, there was a marked increase in astroglial cell proliferation within the SVZ, as assessed by 5-bromodeoxyuridine (BrdU) incorporation in S-phase cells. One month after BrdU incorporation, 40% more BrdU-positive cells were found in the cerebral cortex of hypoxic-reared as compared to normoxic control mice. Among these newly generated cortical cells, approximately 45% were oligodendrocytes, 35% were astrocytes, and 10% were neurons in both hypoxic and normoxic mice. However, twice as many BrdU-labeled cells expressed neuronal markers in the neocortex in mice recovering from hypoxia as compared to controls. In both hypoxic-reared and normoxic infant/juvenile mice, putative neuroblasts could be seen detaching from the forebrain subventricular zone, migrating through the subcortical white matter and entering the lower cortical layers, 5 to 11 days after their last mitotic division. We suggest that cortical neurogenesis may play a significant role in repairing neuronal losses after neonatal injury. (c) 2005 Elsevier Inc. All rights reserved.