THE RO SMALL CYTOPLASMIC RIBONUCLEOPROTEINS - IDENTIFICATION OF THE ANTIGENIC PROTEIN AND ITS BINDING-SITE ON THE RO RNAS
THE RO SMALL CYTOPLASMIC RIBONUCLEOPROTEINS - IDENTIFICATION OF THE ANTIGENIC PROTEIN AND ITS BINDING-SITE ON THE RO RNAS
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DOI：
10.1073/pnas.81.7.1996
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
STEITZ, JA
中科院分区：
文献类型：
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作者：
WOLIN, SL;STEITZ, JA
患有系统性红斑狼疮和其他风湿性疾病的患者通常拥有针对不同类别的小核糖核蛋白颗粒 (RNP) 的自身抗体。抗 Ro 抗体识别的颗粒类别包含 2 到 4 个小细胞质 RNA，具体取决于所检查的哺乳动物物种。 60 kDa [千道尔顿]的抗原性多肽是人 HeLa [人宫颈癌]细胞 Ro RNP 中的主要蛋白质。为了确定 Ro 蛋白识别 Ro RNA 序列或结构的共同特征，对从 HeLa 细胞中分离的 Ro RNP 进行了核糖核酸酶保护实验。对于序列已知的 3 种人类 Ro RNA 中的每一种，在免疫沉淀物中发现的最高度保护的部分对应于通过将 RNA 的 5'' 和 3'' 末端碱基配对形成的茎的下部部分。在这个受保护的螺旋内有一个高度保守的区域，由 7 个相同的碱基对和一个凸出的胞苷组成。 讨论了 Ro RNP 的可能功能。
Patients with systemic lupus erythematosus and other rheumatic diseases often possess autoantibodies directed against discrete classes of small ribonucleoprotein particles (RNP). The class of particles recognized by anti-Ro antibodies contains from 2 to 4 small cytoplasmic RNA, depending on the mammalian species examined. An antigenic polypeptide of 60 kDa [kilodalton] is the major protein residing in Ro RNP from human HeLa [human cervical carcinoma] cells. To determine what common feature of Ro RNA sequence or structure is recognized by the Ro protein, ribonuclease protection experiments were carried out on isolated Ro RNP from HeLa cells. For each of the 3 human Ro RNA whose sequence is known, the most highly protected portion found in immunoprecipitates corresponded to the lower section of a stem formed by base-pairing the 5'' and 3'' ends of the RNA. Within this protected helix is a highly conserved region composed of 7 identical base pairs with a single bulged cytidine. Possible functions for the Ro RNP are discussed.