HELA-LAV, AN EPITHELIAL-CELL LINE STABLY INFECTED WITH HIV-1
HELA-LAV, AN EPITHELIAL-CELL LINE STABLY INFECTED WITH HIV-1
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DOI：
10.1016/0166-0934(91)90097-j
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发表时间：
1991-09-01
影响因子：
3.1
通讯作者：
WABL, M
中科院分区：
文献类型：
--
作者：
BERG, J;DOE, B;WABL, M
通过用HIV-1感染和亚克隆先前描述的表达CD 4的HeLa细胞来建立HeLa-LAV细胞系。 该细胞系的细胞稳定地合成所有主要的HIV蛋白，释放HIV-1的感染性颗粒，但即使在长期培养后也不会死亡。 超过90%的细胞在表面表达包膜蛋白gp 120。 细胞可以容易和有效地用51铬标记，并表现出低的自发释放。 由于它们在靶向gp 120时易被同种异体细胞毒性T细胞（CTL）杀死，因此它们应该是任何类型的HIV特异性杀伤测定中有用的靶细胞来源。 这些细胞也可能有助于研究艾滋病毒在非淋巴细胞/非单核细胞中的复制。 HeLa-LAV细胞系将从艾滋病研究和参考试剂计划免费获得。
An HeLa-LAV cell line was established by infecting and subcloning previously described CD4-expressing HeLa cells with HIV-1. Cells of this line stably synthesize all major HIV proteins, release infectious particles of HIV-1, but do not die even after long term culture. More than 90% of the cells express the envelope protein gp120 on the surface. The cells can be easily and efficiently labeled with 51chromium, and exhibit a low spontaneous release. Because they are susceptible to killing by allogeneic cytotoxic T cells (CTL) when targeted to gp120, they ought to be a useful source of target cells in any kind of HIV-specific killing assays. The cells may also help studies on HIV replication in non-lymphatic/non-monocytic cells. The HeLa-LAV cell line will be freely available from the AIDS Research and Reference Reagent Program.