Premature senescence and cellular phenotype transformation of mesangial cells induced by TGF-B1
Premature senescence and cellular phenotype transformation of mesangial cells induced by TGF-B1
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DOI：
10.3109/0886022x.2013.817279
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发表时间：
2013-01-01
期刊：
影响因子：
3
通讯作者：
Wei, Lin-Ting
中科院分区：
文献类型：
--
作者：
Fu, Rong-Guo;Wu, Juan-Juan;Wei, Lin-Ting
背景资料：转化生长因子-β 1（TGF-β 1）是细胞因子的转化生长因子β超家族的多肽成员，并执行许多细胞功能。其过度表达可导致肾纤维化。目的：探讨TGF-β 1对肾小球系膜细胞周期和表型的影响。研究方法：将大鼠肾小球系膜细胞与不同浓度（0、1、2、5和10 ng/mL）的TGF-β 1一起培养0 min至72 h的指定时间。0 ng/mL TGF-β 1和0 min作为对照。通过流式细胞术评估细胞周期，并通过蛋白质印迹分析评估α-平滑肌肌动蛋白表达（α-SMA）蛋白表达。所有数据均表示为平均值+/- SD。采用单因素方差分析和相关分析进行统计学分析。结果被认为在p
Background: Transforming growth factor-beta 1 (TGF-beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines and performs many cellular functions. Its overexpression may lead to renal fibrosis. Aim: This study planed to investigate the effects of TGF-beta 1 on the cell cycle and phenotype of mesangial cells. Methods: Rat mesangial cells were cultured together with different concentrations (0, 1, 2, 5, and 10 ng/mL) of TGF-beta 1 for specified times from 0 min to 72 h. 0 ng/mL TGF-beta 1 and 0 min served as controls. Cell cycles were assessed by flow cytometry and a-smooth muscle actin expression (alpha-SMA) protein expression by western blot analysis. All data were presented as Mean +/- SD. Statistical analysis was performed by using one-way analysis of variance and correlation analysis. Results were considered significant at p