ISOLATION OF AN AMYLOSE-FREE STARCH MUTANT OF THE POTATO (SOLANUM-TUBEROSUM-L)
ISOLATION OF AN AMYLOSE-FREE STARCH MUTANT OF THE POTATO (SOLANUM-TUBEROSUM-L)
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DOI：
10.1007/bf00249167
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发表时间：
1987-01-01
影响因子：
5.4
通讯作者：
FEENSTRA, WJ
中科院分区：
文献类型：
--
作者：
HOVENKAMPHERMELINK, JHM;JACOBSEN, E;FEENSTRA, WJ
在筛选 12,000 个微型薯后，分离出无直链淀粉的马铃薯突变体。这些微型块茎是在不定芽的茎段上诱导的，在伦琴辐照后，不定芽的茎段在单倍体马铃薯克隆的叶外植体上再生。该突变特征也在地下块茎和小孢子中表达。来自突变体的淀粉颗粒显示颗粒结合淀粉合酶的活性显着降低，并且淀粉颗粒中主要的 60 kd 蛋白质丢失。
An amylose-free potato mutant was isolated after screening 12,000 minitubers. These minitubers had been induced on stem segments of adventitious shoots, which had been regenerated on leaf explants of a monoploid potato clone after Rontgen-irradiation. The mutant character is also expressed in subterranean tubers and in microspores. Starch granules from the mutant showed a strongly reduced activity of the granule bound starch synthase and loss of the major 60 kd protein from the starch granules.