Structural, functional and hybridization studies of the glutathione S-transferases of rat liver.
Structural, functional and hybridization studies of the glutathione S-transferases of rat liver.
复制标题
大鼠肝脏谷胱甘肽 S-转移酶的结构、功能和杂交研究。
DOI：
10.1016/0006-2952(83)90048-5
复制
发表时间：
1983
影响因子：
5.8
通讯作者：
D. Zakim
中科院分区：
文献类型：
--
作者：
T. Boyer;W. Kenney;D. Zakim
We have purified five forms of glutathioneS-transferase from rat liver. One form was the glutathioneS-transferase B (ligandin), which is composed of two non-identical subunits with molecular weights of 22,000 (Ya) and 25,000 (Yc). Two of the other transferases were Ya and Yc homodimers. The other two transferases were also homodimers, but their subunit, Yb, had a molecular weight of 24,000. The three proteins containing either Ya or Yc subunits had similar substrate specificities, and all three contained peroxidase activity. The greatest peroxidase activity was present in proteins containing the Yc subunit. Enzymes composed of Yb subunits had minimal peroxidase activity in addition to different substrate specificities. The Ya and Yc containing enzymes bound the ligands bilirubin and indocyanine green with high affinity (KD< 5μM), although theKDvalues of the YcYc protein were consistently 4- to 12-fold greater than those of the other two transferases. Studies were performed to define the origins of the various isozymes. There was no evidence for conversion of Yc to either Ya or Yb during storage or under conditions favorable to proteolysis. Hybridization studies were performed under denaturing conditions (6 M guanidine-HCl), and a YaYc hybrid was formed from the YaYa and YcYc proteins. In addition, both YaYa and YcYc hybrids were formed from transferase B. The hybrids were functionally similar to the proteins isolated originally from the liver. Attempts to form a YaYb hybrid from the YbYb and YaYa transferases were unsuccessful. This result is consistent with the lack of this enzyme form in the liver. GlutathioneS-transferase B and the Ya and Yc homodimers appeared to be hybrids of common subunits. These three transferases had very similar functional and structural characteristics and differed from the transferases that are composed of Yb subunits.
大鼠谷胱甘肽 S-转移酶。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kalinyak,JE;Taylor,JM
通讯作者：
Taylor,JM