Expression of Merkelcell polyomavirus (MCPyV) large T-antigen in Merkel cell carcinoma lymph node metastases predicts poor outcome
Expression of Merkelcell polyomavirus (MCPyV) large T-antigen in Merkel cell carcinoma lymph node metastases predicts poor outcome
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DOI：
10.1371/journal.pone.0180426
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发表时间：
2017-08-01
期刊：
影响因子：
3.7
通讯作者：
Erovic, Boban M.
中科院分区：
文献类型：
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作者：
Haymerle, Georg;Janik, Stefan;Erovic, Boban M.
本研究的目的是确定MCPyV在Merkel细胞癌(MCC)原发灶和淋巴结转移灶中的患病率，并评价可能的预后因素。方法采用免疫组织化学方法检测MCC原发灶和淋巴结转移灶标本中MCPyV大T抗原的表达，并与A组临床结果进行比较。54例标本中33例(61%)MCPyV免疫组化阳性。32个原发灶中15个阳性(47%)，而22个淋巴结转移灶中18个阳性(82%)。11例多瘤病毒表达阳性的患者死于癌症，而4例无病毒表达的患者死于癌症。COX回归分析显示，MCPyV阳性患者的无瘤生存率比病毒阴性患者差(p=0.002)。结论本研究首次描述了MCPyV表达对MCC患者淋巴结转移的负面预后影响。
BackgroundThe aim of this study was to determine the prevalence of MCPyV in Merkel cell carcinoma (MCC) primaries versus lymph node metastasis and to evaluate possible prognostic factors.MethodsSamples of MCC primaries and lymph node metastases were stained immunohistochemically for the MCPyV large T-antigen and expression was compared to patients A clinical outcome.Results41 MCC patients were included. 33 (61%) out of 54 specimens were MCPyV-positive in the immunohistochemistry. 15 (47%) out of 32 primary tumors were positive compared to 18 (82%) out of 22 lymph node metastases. Eleven patients with positive polyomavirus expression died from the carcinoma compared to 4 patients without virus expression. Cox regression analysis showed worse disease-free survival in patients with MCPyV compared to virus-negative lymph nodes (p = 0.002).ConclusionsTo our knowledge this is the first study to describe a negative prognostic effect of the MCPyV expression in lymph node metastasis in MCC patients.