Using dense locality sampling resolves the subtle genetic population structure of the dispersive fish species Plecoglossus altivelis.
Using dense locality sampling resolves the subtle genetic population structure of the dispersive fish species Plecoglossus altivelis.
复制标题
使用密集局部采样解决了分散性鱼类 Plecoglossus altivelis 的微妙遗传种群结构。
DOI：
10.1111/mec.13650
复制
发表时间：
2016
影响因子：
4.9
通讯作者：
Mutsumi Nishida
中科院分区：
文献类型：
--
作者：
Hirohiko Takeshima;Kei'ichiro Iguchi;Yasuyuki Hashiguchi;Mutsumi Nishida
在具有连续分布的分散物种中，当地种群之间的遗传分化往往是不存在的或微妙的，因此难以检测。为了将这种微妙的差异纳入管理计划，可能必须使用足够数量的高分辨率遗传标记分析来自许多地方的许多样品。在这里，我们评估了密集的地方采样在解决遗传种群结构的香鱼（Plecoglossus altivelis），分散的鱼在日本内陆渔业的重要作用。通过对12个微卫星标记进行基因分型，研究了日本-琉球群岛附近香鱼种群的遗传变异和种群间的分化。这些个体代表了香鱼的两个亚种，即琉球亚种（Plecoglossus altivelis ryukyuensis）和沿着群岛的两种两栖和内陆形式的命名亚种（P. a. altivelis）。我们成功地检测到没有遗传分化的内陆形式和微妙的，但显着的分化和明确的地理模式的遗传变异种群间的amphirromous形式，这一直被认为是遗传同质的。这表明，密集的地方采样有效地解决了遗传种群结构的细微差异，减少随机偏差的遗传分化和地理分布在当地种群的这种分散的物种的检测。基于经验数据集的恢复分析清楚地表明，增加当地样本数量的有效性，稳定和可靠的遗传固定指数的估计。遗传种群结构内观察到的无转形式提供了有用的信息，以确定管理或保护单位香鱼。
In dispersive species with continuous distributions, genetic differentiation between local populations is often absent or subtle and thus difficult to detect. To incorporate such subtle differentiation into management plans, it may be essential to analyse many samples from many localities using adequate numbers of high‐resolution genetic markers. Here, we evaluated the usefulness of dense locality sampling in resolving genetic population structure in the ayu (Plecoglossus altivelis), a dispersive fish important in Japanese inland fisheries. Genetic variability in, and differentiation between, ayu populations around the Japan–Ryukyu Archipelago were investigated in 4746 individuals collected from 120 localities by genotyping 12 microsatellite markers. These individuals represented the two subspecies of ayu, namely the Ryukyuan subspecies (Plecoglossus altivelis ryukyuensis) and both amphidromous and landlocked forms of the nominotypical subspecies (P. a. altivelis) along the archipelago. We successfully detected an absence of genetic differentiation within the landlocked form and subtle but significant differentiation and clear geographic patterns of genetic variation among populations of the amphidromous form, which had been considered genetically homogeneous. This suggests that dense locality sampling effectively resolves subtle differences in genetic population structure, reducing stochastic deviation in the detection of genetic differentiation and geographic patterns in local populations of this dispersive species. Resampling analyses based on empirical data sets clearly demonstrate the effectiveness of increasing the number of locality samples for stable and reliable estimations of genetic fixation indices. The genetic population structure observed within the amphidromous form provides useful information for identifying management or conservation units in ayu.