mRNA stability plays a major role in regulating the temperature-specific expression of a Tetrahymena thermophila surface protein.
mRNA stability plays a major role in regulating the temperature-specific expression of a Tetrahymena thermophila surface protein.
复制标题
mRNA 稳定性在调节嗜热四膜虫表面蛋白的温度特异性表达中起着重要作用。
DOI：
10.1128/mcb.8.1.427-432.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
Bannon,GA
中科院分区：
文献类型：
--
作者：
LoveJr,HD;Allen-Nash,A;Zhao,QA;Bannon,GA
Synthesis of the serotype H3 (SerH3) surface antigen is temperature dependent and responds within 1 h to a change in incubation conditions (G.A. Bannon, R. Perkins-Dameron, and A. Allen-Nash, Mol. Cell. Biol. 6:3240-3245, 1986). Recently, aTetrahymena thermophilacDNA clone (pC6; D.W. Martindale and P.J. Bruns, Mol. Cell. Biol. 3:1857-1865, 1983) has been shown to be homologous to a portion of the SerH3 mRNA (F.P. Doerder and R.L. Hallberg, personal communication), and it was shown that the cellular levels of this RNA rapidly decreased when cells were shifted from 30 to 41°C (R.L. Hallberg, K.W. Kraus, and R.C. Findly, Mol. Cell. Biol. 4:2170-2179, 1984). These observations indicate that synthesis of the SerH3 protein is highly regulated in response to temperature and led us to initiate studies to determine the mechanism(s) by which SerH3 gene expression is controlled. Using pC6 as a hybridization probe for the SerH3 mRNA, we have determined that (i) the level of SerH3 protein synthesis is directly correlated with the amount of SerH3 message available for translation; (ii) there is, at most, a twofold difference between the relative transcription rates of SerH3 genes at 30 and 40°C; (iii) the SerH3 mRNA half-life in cells incubated at 30°C is greater than 1 h, whereas the half-life in cells incubated at 40°C is only ~3 min. These results demonstrate thatTetrahymenaSerH3 surface protein expression is regulated by mRNA abundance. Furthermore, the major mechanism controlling mRNA abundance is a dramatic temperature-dependent change in SerH3 mRNA stability.
