A human protein atlas for normal and cancer tissues based on antibody proteomics
A human protein atlas for normal and cancer tissues based on antibody proteomics
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DOI：
10.1074/mcp.m500279-mcp200
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发表时间：
2005-12-01
影响因子：
7
通讯作者：
Pontén, F
中科院分区：
文献类型：
--
作者：
Uhlén, M;Björling, E;Pontén, F
基于抗体的蛋白质组学为人类蛋白质组的功能研究提供了一个强有力的方法，包括系统地产生蛋白质特异性亲和试剂。我们使用这种策略构建了一个全面的，基于抗体的蛋白质图谱，用于48种正常人体组织和20种不同癌症的表达和定位谱。在这里，我们报告了一个新的公开可用的数据库，在第一个版本中，包含类似于400，000个高分辨率图像，对应于700多个针对人类蛋白质的抗体。每张图像都由经过认证的病理学家进行注释，为功能研究提供知识库，并允许查询正常和疾病组织中的蛋白质谱。我们的研究结果表明，应该有可能将这种分析扩展到大多数人类蛋白质，从而为医学和生物学研究提供有价值的工具。
Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.