Exploring diversity in cryptorhynchine weevils (Coleoptera) using distance-, character- and tree-based species delineation
Exploring diversity in cryptorhynchine weevils (Coleoptera) using distance-, character- and tree-based species delineation
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DOI：
10.1016/j.ympev.2011.11.018
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发表时间：
2012-04-01
影响因子：
4.1
通讯作者：
Ahrens, Dirk
中科院分区：
文献类型：
--
作者：
Astrin, Jonas J.;Stueben, Peter E.;Ahrens, Dirk
物种边界研究了一组甲虫，西部古北隐蝽科。我们测试了“传统”识别的形态物种与通过简约网络、基于距离的聚类和基于超密树的广义混合yul -coalescent （GMYC）方法推断的物种的一致性。为此，我们对217种隐蝽科（Cryptorhynchinae） 791个标本的两个可变线粒体DNA片段（CO1和16S）进行了测序。简约网络、形态校准的距离聚类和不同树为基础的物种推断都与形态物种的一致性较低，最多为60%。虽然不同的方法与形态种的匹配程度往往相似，但簇的组成部分不同。在形态物种导向距离和GMYC物种集群中不存在条形码间隙。这表明，不仅错误的分类分配、不完整的谱系分类、杂交或采样不足会损害基于距离的鉴定，而且还会影响物种形成率的差异和树结构的不均匀。在本案例研究中，形态物种与不同分子物种描述方法之间最初的低匹配表明，有必要将各种方法的输出结合在一个综合方法中。因此，我们对不同结果和信号的可靠性有了一个概念，这使我们能够微调采样，描绘技术和数据收集，并确定需要分类修订的物种。(C) 2011爱思唯尔公司版权所有。
Species boundaries are studied in a group of beetles, the western Palaearctic Cryptorhynchinae. We test for congruence of 'traditionally' identified morphospecies with species inferred through parsimony networks, distance-based clustering and the ultrametric tree-based generalized mixed yule-coalescent (GMYC) approach. For that purpose, we sequenced two variable fragments of mitochondrial DNA (CO1 and 16S) for a total of 791 specimens in 217 species of Cryptorhynchinae. Parsimony networks, morphology-calibrated distance clusters and the different tree-based species inferences all achieved low congruence with morphospecies, at best 60%. Although the degree of match with morphospecies was often similar for the different approaches, the composition of clusters partially varied. A barcoding gap was absent in morphospecies-oriented distances as well as for GMYC species clusters. This demonstrates that not only erroneous taxonomic assignments, incomplete lineage sorting, hybridization, or insufficient sampling can compromise distance-based identification, but also differences in speciation rates and uneven tree structure. The initially low match between morphospecies and the different molecular species delineation methods in this case study shows the necessity of combining the output of various methods in an integrative approach. Thereby we obtain an idea about the reliability of the different results and signals, which enables us to fine-tune sampling, delineation technique and data collection, and to identify species that require taxonomic revision. (C) 2011 Elsevier Inc. All rights reserved.