Hepatic proteomic assessment of oral ingestion of titanium dioxide nano fiber (TDNF) in Sprague Dawley rats
Hepatic proteomic assessment of oral ingestion of titanium dioxide nano fiber (TDNF) in Sprague Dawley rats
复制标题
DOI：
10.1080/10934529.2022.2159733
复制
发表时间：
2023-01-02
影响因子：
2.1
通讯作者：
Rochani, Haresh
中科院分区：
文献类型：
--
作者：
Gato, Worlanyo E.;Wu, Ji;Rochani, Haresh
二氧化钛纳米纤维（TDNF）已被广泛应用于颜料、防晒剂、油漆、软膏、牙膏和光催化分解水。然而，其潜在的毒性尚未得到彻底的研究。本研究的目的是检查与摄入TDNF相关的肝脏效应。采用静电纺丝法制备了TDNF，并对其进行了表征。六至七周龄的雄性Sprague道利大鼠摄入（经口管饲）总共0 ppm、40、60 ppm的TDNF两周。处死后，使用蛋白质组学方法评估肝脏的细胞效应。纤维直径为0.18 - 0.29 μ m，形成团簇，大部分纤维为金红石相。蛋白质组学评估显示，肝脏中超过400种蛋白质可能受到影响。这些蛋白质参与诸如CoA催化脂肪酸、高半胱氨酸代谢、β氧化和尿素循环中氨基甲酰基磷酸缩合等过程。对蛋白质关联的进一步分析表明，325个生物过程、140个分子功能和70个细胞组分似乎受到TNDF摄入的影响。特异性mRNA转录本定量分析显示CMBL、GSTM 1和SDS的差异表达。
Titanium dioxide nanofibers (TDNF) have been widely employed in pigments, sunscreens, paints, ointments, toothpaste and photocatalytic splitting of water. However, their potential toxicity has not been thoroughly examined. The goal of the present study is to examine hepatic effects associated with the ingestion of TDNF. TDNF was fabricated via electrospinning method and characterized. Six to seven weeks old male Sprague Dawley rats ingested (oral gavage) a total of 0 ppm, 40, 60 ppm TDNF for two weeks. After sacrifice, the liver was assessed for cellular effects using proteomic approach. The fibers diameter ranged from 0.18 - 0.29 mu m, forming clusters and majority of the fibers were in the rutile phase. Proteomics assessment revealed more that more than 400 hundred proteins in the liver may be affected. These proteins are involved in such processes as catalysis of fatty acids by CoA, homocysteine metabolism, beta oxidation and the condensation of carbamoyl phosphate in the urea cycle among others. Further analysis of the protein associations showed that 325 biological processes, 140 molecular functions and 70 cellular components appear to be affected from the ingestion of TNDF. Quantitative analysis of specific mRNA transcripts indicated CMBL, GSTM1 and SDS were differentially expressed.