Determination of biofilm production by Candida tropicalis isolated from hospitalized patients and its relation to cellular surface hydrophobicity, plastic adherence and filamentation ability
Determination of biofilm production by Candida tropicalis isolated from hospitalized patients and its relation to cellular surface hydrophobicity, plastic adherence and filamentation ability
复制标题
DOI：
10.1002/yea.2965
复制
发表时间：
2013-09-01
期刊：
影响因子：
2.6
通讯作者：
Gomez-Garcia, A. C.
中科院分区：
文献类型：
--
作者：
Galan-Ladero, M. A.;Blanco-Blanco, M. T.;Gomez-Garcia, A. C.
热带念珠菌是一种新兴的毒力极强的菌种。本研究旨在测定29株从住院患者身上分离的热带梭菌的生物膜形成能力，并探讨其与细胞表面疏水性（CSH）、即时（15 min， IA）和后期（24 h， LA）的塑料粘附和成丝能力等毒力因素的关系。该研究采用两种孵育温度（37℃和22℃）并行进行，以确定生长温度变化对热带镰刀菌这些致病特性的影响。采用光密度法（OD）和XTT还原法（XTT）测定生物膜形成（BF）；两种方法均计算了含生长因子的煤泥指数（SI）。在两种温度下，所有菌株在15 min和24 h均具有疏水性和粘附性，在22℃时具有较高的粘附性；粘附的基础酵母菌层似乎是实现生物膜后续发展所必需的。37℃时成丝率为76.2%，22℃时成丝率为26.6%，所有菌株都是生物膜的产生者，OD测定和XTT测定结果与评价方法相似；当没有出现良好的增长时，SI是有用的。37℃孵育24 h和96 h时BF相似；相反，在22℃时，96 h产膜菌数量最多。CSH是一种重要的致病因子，它参与粘附，受酵母成丝的影响，在BF中起关键作用。版权所有：John Wiley & Sons， Ltd。
Candida tropicalis is an emerging virulent species. The aim of this study is to determine the biofilm-forming ability of 29 strains of C. tropicalis isolated from inpatients, and to examine its relation with other virulence factors such as cellular surface hydrophobicity (CSH), immediate (15 min, IA) and late (24 h, LA) plastic adherence and filamentation ability. The study was performed in parallel using two incubation temperatures - 37 and 22 degrees C - to determine the effect of growth temperature variations on these pathogenic attributes of C. tropicalis. Biofilm formation (BF) was measured by optical density (OD) and by XTT reduction (XTT); Slime index (SI), which includes growth as a correction factor in BF, was calculated in both methods. All strains were hydrophobic and adherent - at 15 min and 24 h - at both temperatures, with higher values for 22 degrees C; the adhered basal yeast layer appears to be necessary to achieve subsequent development of biofilm. Filamentation ability varied from 76.2% of strains at 37 degrees C to 26.6% at 22 degrees C. All C. tropicalis strains were biofilm producers, with similar results obtained using OD determination and XTT measurement to evaluation methods; SI is useful when good growth is not presented. BF at 37 degrees C was similar at 24 h and 96 h incubation; conversely, at 22 degrees C, the highest number of biofilm-producing strains was detected at 96 h. CSH is an important pathogenic factor which is involved in adherence, is influenced by the filamentation of yeast, and plays a critical role in BF. Copyright (c) 2013 John Wiley & Sons, Ltd.