Cell Membrane Permeabilization Studies of Chlorella sp. by Pulsed Electric Fields
Cell Membrane Permeabilization Studies of Chlorella sp. by Pulsed Electric Fields
复制标题
小球藻的细胞膜透化研究。
DOI：
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发表时间：
2015
影响因子：
1.5
通讯作者：
Luis Redondo
中科院分区：
文献类型：
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作者：
D. Rego;L. Costa;M. Pereira;Luis Redondo
研究了微藻细胞膜的透性，对于小球藻，在不同场强的连续6 L原型中，以最小化供应的比能量和治疗时间。关于较低的电场强度值，在10 kV/cm、200 Hz和5 μs/脉冲下获得了最佳结果，对应于9 kJ/kg培养物的能量输送，在300 ms的处理时间后97%的细胞受到影响。关于每千克生物质递送的能量，最好的结果是2.7 MJ/kg生物质，在300 ms的处理时间后95%的细胞受到影响，并且在15 kV/cm、200 Hz和5 μs/脉冲下实现。使用碘化丙啶作为活力染色剂通过流式细胞术定量脉冲电场效应。并分析了电导率和细胞浓度对细胞生长的影响。最终目标是评估该技术在提高微藻中生物活性化合物的生物可及性方面的用途，以用于食品和饲料目的。这些结果仅针对叶绿素a和B获得，没有显示该技术对未处理的生物质的任何有益效果。
Microalgae cell membrane permeabilization has been studied, for Chlorella sp., in a continuous 6-L prototype for different field strengths in order to minimize the specific energy supplied and the treatment time. Regarding lower electrical field strength values, the optimal results were achieved at 10 kV/cm, 200 Hz, and 5-μs per pulse, corresponding to an energy delivered of 9 kJ/kg of culture, with 97% of cells affected after 300 ms of treatment time. Regarding energy delivered per kilogram of Biomass, the best result was 2.7 MJ/kg of biomass with 95% of cells affected after 300 ms of treatment time and was achieved at 15 kV/cm, 200 Hz, and 5-μs per pulse. Pulsed electric field effect was quantified by flow cytometry using propidium iodide as a viability stain. The influence of conductivity and cell concentration was also analyzed. The final goal was to evaluate the use of this technology in terms of increasing bioaccessibility of bioactive compounds present in microalgae for food and feed purposes. These results, obtained only for chlorophyll a and b, did not show any beneficial effect of this technology against nontreated biomass.
影响因子：
11.4
作者：
Biller, Patrick;Friedman, Cerri;Ross, Andrew B.
通讯作者：
Ross, Andrew B.