The N-terminal domain of the rne gene product has RNase E activity and is non-overlapping with the arginine-rich RNA-binding site
The N-terminal domain of the rne gene product has RNase E activity and is non-overlapping with the arginine-rich RNA-binding site
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DOI：
10.1006/jmbi.1996.0027
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发表时间：
1996-01-26
影响因子：
5.6
通讯作者：
Cohen, SN
中科院分区：
文献类型：
--
作者：
McDowall, KJ;Cohen, SN
大肠杆菌的rne基因编码具有核糖核酸酶E（RNase E）活性并结合RNA的118 kDa蛋白。功能性I ne基因产物对于细胞活力和对于多种RNA种类的加工和/或降解是必需的，所述RNA种类包括9 S RNA、mRNA和RNAi（ColE 1型质粒复制的反义RNA调节剂）。通过测试Rne蛋白的不同片段催化RNA切割和结合RNA的能力，我们发现Rne的N-末端一半（残基1至498）包含足以用于寡核糖核苷酸和复杂RNA的位点特异性切割的催化功能。蛋白质的C-末端的一半，其中包含一个富含精氨酸的区域（残基597至684），我们显示结合RNA和一个片段，这是细胞活力所必需的（残基844至1061），没有检测到的endoribonucleolytic活性我们的结果，它映射的催化结构域的RNase E，表明存在的离散的功能结构域内的多方面的Rne蛋白。(C)1996年学术出版社
The rne gene of Escherichia coli encodes a 118 kDa protein that has ribonuclease E (RNase E) activity and binds RNA. A functional I ne gene product is essential for cell viability and for the processing and/or decay of a variety of RNA species, including 9 S RNA, mRNA and RNAI, the antisense RNA regulator of ColE1-type plasmid replication. By testing the ability of different segments of the Rne protein to catalyze RNA cleavage and to bind RNA, we found that the N-terminal half (residues 1 to 498) of Rne contains a catalytic function sufficient for site-specific cleavage of oligoribonucleotides and complex RNAs. The C-terminal half of the protein, which contains both an arginine-rich region (residues 597 to 684) that we show binds RNA and a segment that is essential for cell viability (residues 844 to 1061), had no detectable endoribonucleolytic activity Our results, which map the catalytic domain of RNase E, indicate the existence of discrete functional domains within the multifaceted Rne protein. (C) 1996 Academic Press Limited