Characterization of a putative retroviral env-related human protein.
Characterization of a putative retroviral env-related human protein.
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假定的逆转录病毒环境相关人类蛋白的表征。
DOI：
10.1159/000164113
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Steele,PE
中科院分区：
文献类型：
--
作者：
Turbeville,MA;Rhodes,JC;Hyams,DM;Distler,CM;Steele,PE
在这项研究中，我们开发了一种兔多克隆抗体的融合蛋白含有包膜（env）跨膜（TM）区从人类内源性逆转录病毒家族，HERV-E。我们使用该试剂通过Western印迹法记录TM相关蛋白在内皮细胞、结肠癌和前列腺癌细胞系以及来自健康供体的外周血单核细胞中的表达。我们检测到一个58-kD的蛋白质（与小鼠TM的15 kD相比），具有特异性的多克隆抗血清的环境相关抗体。
In this study, we developed a rabbit polyclonal antibody to a fusion protein containing the envelope (env) transmembrane (TM) region from the human endogenous retroviral family, HERV-E. We used this reagent to document the expression of TM-related protein by Western blot in endothelial, colon and prostate carcinoma and seminoma cell lines and in peripheral blood mononuclear cells from a healthy donor. We detected a 58-kD protein (as compared to murine TM of 15 kD) that had specificity for theenv-related antibodies of the polyclonal antiserum.