Hydrodynamic micromanipulation of individual cells onto patterned attachment sites on biomicroelectromechanical system chips.
Hydrodynamic micromanipulation of individual cells onto patterned attachment sites on biomicroelectromechanical system chips.
复制标题
将单个细胞流体动力学显微操作到生物微机电系统芯片上的图案化附着位点上。
DOI：
10.1021/ac030055u
复制
发表时间：
2003
影响因子：
7.4
通讯作者：
M. Gratzl
中科院分区：
文献类型：
--
作者：
Makoto Yoshida;K. Tohda;M. Gratzl
我们报告了一种简单的方法，将选定的单个活细胞移动到平板衬底上的所需位置，例如图案化的生物微电子机械系统芯片。基于宏观注射器和管子的流体动力学操作系统被用来实现受控的细胞导航到亲水部位以进行细胞附着。由于介质中的粘性流动，系统产生的厘米/秒的流动速度在固定单元的高度被降低到微米/秒的流动。通过推/拉两个注射器产生两个正交流，在任何水平方向上的精细操作都是可行的。将所需细胞(S)附着到选定的亲水部位后，所有其他不需要的细胞以更快的速度从周围的疏水表面被冲走。这种简单的方法适用于高精度的快速细胞图案形成。
We report on a simple methodology to move selected single live cells to a desired location on a flat substrate, such as a patterned biomicroelectromechanical system chip. A macroscopic syringe-and-tube-based hydrodynamic manipulation system is used to achieve controlled cell navigation onto hydrophilic sites for cell attachment. Centimeter-per-second flow velocities generated by the system get downgraded to micrometers-per-second flow at the height of settled cells as a result of viscous flow in the medium. By pushing/pulling two syringes that produce two orthogonal flows, fine manipulation in any horizontal direction is feasible. After attachment of the desired cell(s) onto the selected hydrophilic site, all other unwanted cells are washed away from the surrounding hydrophobic surface with faster flow. This simple methodology is applicable for rapid cell pattern formation with high precision.