Microbial transformation of virus-induced dissolved organic matter from picocyanobacteria: coupling of bacterial diversity and DOM chemodiversity
Microbial transformation of virus-induced dissolved organic matter from picocyanobacteria: coupling of bacterial diversity and DOM chemodiversity
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DOI：
10.1038/s41396-019-0449-1
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发表时间：
2019-10-01
期刊：
影响因子：
11
通讯作者：
Chen, Feng
中科院分区：
文献类型：
--
作者：
Zhao, Zhao;Gonsior, Michael;Chen, Feng
花青菌占海洋初级产品的一半，它们经常受到病毒感染。细菌的病毒裂解是将生物固定碳转化为溶解有机碳（DOC）的主要驱动力。微蓝杆菌释放的病毒诱导溶解有机物（vDOM）为海洋生态系统中的浮游细菌提供了复杂的有机物。为了了解细菌对piccyanobacterian vDOM的转化过程以及这一过程对细菌群落结构的影响，我们将piccyanobacterian的病毒裂解液与沿海海水孵育90天。利用超高分辨率质谱分析vDOM的转化，利用高通量测序技术分析细菌群体的转移。picocyanbacterium vDOM的加入为沿海水体引入了丰富的氮成分，在90天的孵育期内，这些氮成分大部分被降解。但是，累积了一些DOM签名，并且分配的公式总数随着时间的推移而增加。与未添加vDOM的对照组相比，随着生物多样性指数的增加，富含vDOM的细菌群落发生了显著变化。网络分析表明，关键菌种与vDOM成分形成了复杂的关系，提示细菌种群与DOM分子之间可能存在对应关系。研究表明，沿海浮游细菌能够快速利用和转化picocyanobacteria的裂解产物，同时细菌群落也随着DOM化学多样性的变化而变化。picocyanobacteria释放的vDOM生成了一个复杂的不稳定DOM池，经过几天到几周的微生物处理后转化为一个相当稳定的DOM池。
Picocyanobacteria make up half of the ocean's primary production, and they are subjected to frequent viral infection. Viral lysis of picocyanobacteria is a major driving force converting biologically fixed carbon into dissolved organic carbon (DOC). Viral-induced dissolved organic matter (vDOM) released from picocyanobacteria provides complex organic matter to bacterioplankton in the marine ecosystem. In order to understand how picocyanobacterial vDOM are transformed by bacteria and the impact of this process on bacterial community structure, viral lysate of picocyanobacteria was incubated with coastal seawater for 90 days. The transformation of vDOM was analyzed by ultrahigh-resolution mass spectrometry and the shift of bacterial populations analyzed using high-throughput sequencing technology. Addition of picocyanobacterial vDOM introduced abundant nitrogen components into the coastal water, which were largely degraded during the 90 days' incubation period. However, some DOM signatures were accumulated and the total assigned formulae number increased over time. In contrast to the control (no addition of vDOM), bacterial community enriched with vDOM changed markedly with increased biodiversity indices. The network analysis showed that key bacterial species formed complex relationship with vDOM components, suggesting the potential correspondence between bacterial populations and DOM molecules. We demonstrate that coastal bacterioplankton are able to quickly utilize and transform lysis products of picocyanobacteria, meanwhile, bacterial community varies with changing chemodiverisity of DOM. vDOM released from picocyanobacteria generated a complex labile DOM pool, which was converted to a rather stable DOM pool after microbial processing in the time frame of days to weeks.