Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification
Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification
复制标题
DOI：
10.1016/j.jsb.2011.12.005
复制
发表时间：
2012-02-01
影响因子：
3
通讯作者：
Cloetens, Peter
中科院分区：
文献类型：
--
作者：
Kosior, Ewelina;Bohic, Sylvain;Cloetens, Peter
硬X射线荧光显微镜和放大的相衬成像相结合，以获得整个细胞内投射的金属浓度的定量地图。这些实验是在欧洲同步辐射设施(ESRF)的纳米成像站ID22NI的冷冻干燥细胞上进行的。X射线荧光分析给出了大多数主要、次要和微量元素的面积质量；它使用已知成分的生物标准进行了验证。定量相衬成像提供了投影质量的地图，并使用校准样品并通过与原子力显微镜和扫描透射式离子显微镜的比较进行了验证。到目前为止，使用X射线荧光显微镜在亚细胞水平上进行绝对定量是不可能的，但可以使用所提出的方法来实现。(C)2011 Elsevier Inc.保留所有权利。
Hard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to obtain quantitative maps of the projected metal concentration in whole cells. The experiments were performed on freeze dried cells at the nano-imaging station ID22NI of the European Synchrotron Radiation Facility (ESRF). X-ray fluorescence analysis gives the areal mass of most major, minor and trace elements; it is validated using a biological standard of known composition. Quantitative phase contrast imaging provides maps of the projected mass and is validated using calibration samples and through comparison with Atomic Force Microscopy and Scanning Transmission Ion Microscopy. Up to now, absolute quantification at the sub-cellular level was impossible using X-ray fluorescence microscopy but can be reached with the use of the proposed approach. (C) 2011 Elsevier Inc. All rights reserved.